C-Phycoerythrin as a Colorimetric and Fluorometric Probe for the Sensitive, Selective and Quantitative Detection of Cu2+ in Aqueous Samples

C-Phycoerythrin as a Colorimetric and Fluorometric Probe for the Sensitive, Selective and... C-phycoerythrin (CPE) was investigated as a colorimetric and fluorometric quantitative sensor for Cu2+ ions in an aqueous medium. UV – visible studies with 50 μM concentration of different metals were carried out with only Cu and Ag showing changes in the absorption spectra. Fluorescence emission studies showed similar results. UV – visible titration of CPE with different [Cu] resulted in a linear relationship within 10 μM Cu and a ‘naked eye’ visible difference in colour, most likely due to the formation of a CPE – Cu complex. Fluorescence emission of CPE was quenched rapidly within 5 min of mixing. Fluorescence emission titration studies revealed gradually decreasing CPE emission with increasing [Cu] with a Stern – Volmer constant of 2.5 × 104 M−1 and a detection limit of 5 μM.. CPE was selective for Cu even in the presence of different metals which were 5 times the concentration of Cu; it was also effective in aqueous samples spiked with Cu. FTIR studies showed considerable changes in the amide III, indicating side chain interactions, even as the protein backbone remained largely unaffected. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fluorescence Springer Journals

C-Phycoerythrin as a Colorimetric and Fluorometric Probe for the Sensitive, Selective and Quantitative Detection of Cu2+ in Aqueous Samples

Loading next page...
 
/lp/springer_journal/c-phycoerythrin-as-a-colorimetric-and-fluorometric-probe-for-the-5znDV4KseE
Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Biomedicine; Biomedicine, general; Biological and Medical Physics, Biophysics; Biotechnology; Biochemistry, general; Analytical Chemistry
ISSN
1053-0509
eISSN
1573-4994
D.O.I.
10.1007/s10895-018-2229-0
Publisher site
See Article on Publisher Site

Abstract

C-phycoerythrin (CPE) was investigated as a colorimetric and fluorometric quantitative sensor for Cu2+ ions in an aqueous medium. UV – visible studies with 50 μM concentration of different metals were carried out with only Cu and Ag showing changes in the absorption spectra. Fluorescence emission studies showed similar results. UV – visible titration of CPE with different [Cu] resulted in a linear relationship within 10 μM Cu and a ‘naked eye’ visible difference in colour, most likely due to the formation of a CPE – Cu complex. Fluorescence emission of CPE was quenched rapidly within 5 min of mixing. Fluorescence emission titration studies revealed gradually decreasing CPE emission with increasing [Cu] with a Stern – Volmer constant of 2.5 × 104 M−1 and a detection limit of 5 μM.. CPE was selective for Cu even in the presence of different metals which were 5 times the concentration of Cu; it was also effective in aqueous samples spiked with Cu. FTIR studies showed considerable changes in the amide III, indicating side chain interactions, even as the protein backbone remained largely unaffected.

Journal

Journal of FluorescenceSpringer Journals

Published: Apr 18, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off