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C-Phycoerythrin as a Colorimetric and Fluorometric Probe for the Sensitive, Selective and Quantitative Detection of Cu2+ in Aqueous Samples

C-Phycoerythrin as a Colorimetric and Fluorometric Probe for the Sensitive, Selective and... C-phycoerythrin (CPE) was investigated as a colorimetric and fluorometric quantitative sensor for Cu2+ ions in an aqueous medium. UV – visible studies with 50 μM concentration of different metals were carried out with only Cu and Ag showing changes in the absorption spectra. Fluorescence emission studies showed similar results. UV – visible titration of CPE with different [Cu] resulted in a linear relationship within 10 μM Cu and a ‘naked eye’ visible difference in colour, most likely due to the formation of a CPE – Cu complex. Fluorescence emission of CPE was quenched rapidly within 5 min of mixing. Fluorescence emission titration studies revealed gradually decreasing CPE emission with increasing [Cu] with a Stern – Volmer constant of 2.5 × 104 M−1 and a detection limit of 5 μM.. CPE was selective for Cu even in the presence of different metals which were 5 times the concentration of Cu; it was also effective in aqueous samples spiked with Cu. FTIR studies showed considerable changes in the amide III, indicating side chain interactions, even as the protein backbone remained largely unaffected. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fluorescence Springer Journals

C-Phycoerythrin as a Colorimetric and Fluorometric Probe for the Sensitive, Selective and Quantitative Detection of Cu2+ in Aqueous Samples

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Biomedicine; Biomedicine, general; Biological and Medical Physics, Biophysics; Biotechnology; Biochemistry, general; Analytical Chemistry
ISSN
1053-0509
eISSN
1573-4994
DOI
10.1007/s10895-018-2229-0
pmid
29667001
Publisher site
See Article on Publisher Site

Abstract

C-phycoerythrin (CPE) was investigated as a colorimetric and fluorometric quantitative sensor for Cu2+ ions in an aqueous medium. UV – visible studies with 50 μM concentration of different metals were carried out with only Cu and Ag showing changes in the absorption spectra. Fluorescence emission studies showed similar results. UV – visible titration of CPE with different [Cu] resulted in a linear relationship within 10 μM Cu and a ‘naked eye’ visible difference in colour, most likely due to the formation of a CPE – Cu complex. Fluorescence emission of CPE was quenched rapidly within 5 min of mixing. Fluorescence emission titration studies revealed gradually decreasing CPE emission with increasing [Cu] with a Stern – Volmer constant of 2.5 × 104 M−1 and a detection limit of 5 μM.. CPE was selective for Cu even in the presence of different metals which were 5 times the concentration of Cu; it was also effective in aqueous samples spiked with Cu. FTIR studies showed considerable changes in the amide III, indicating side chain interactions, even as the protein backbone remained largely unaffected.

Journal

Journal of Fluorescence – Springer Journals

Published: Apr 18, 2018

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