Brassinolides promote the expression of a new Cicer arietinum β-tubulin gene involved in the epicotyl elongation

Brassinolides promote the expression of a new Cicer arietinum β-tubulin gene involved in the... A cDNA clone, CanTUB, encoding a putative β-tubulin protein was isolated from a cDNA library constructed from 5-day old chickpea (Cicer arietinum) epicotyls. Analysis of its deduced amino acid sequence showed all the typical structural motifs of plant β-tubulins. Putative sequences for autoregulation and tubulin mRNA stability, GTP and Ca2+/MAPs (microtubule-associated proteins) binding sites were present. Southern blot analysis of chickpea genomic DNA revealed that there are multiple β-tubulin genes. The level of expression of β-tubulin genes was correlated with the rate of growth in either seedlings and adult plants. The transcript levels of β-tubulin genes were higher in actively elongating tissues such as etiolated epicotyls, roots and stem tissues of adult plants. Brassinolide-induced growth in chickpea epicotyls was accompanied by promotion of the expression of the gene coding for β-tubulin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Brassinolides promote the expression of a new Cicer arietinum β-tubulin gene involved in the epicotyl elongation

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Publisher
Springer Journals
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1006013718429
Publisher site
See Article on Publisher Site

Abstract

A cDNA clone, CanTUB, encoding a putative β-tubulin protein was isolated from a cDNA library constructed from 5-day old chickpea (Cicer arietinum) epicotyls. Analysis of its deduced amino acid sequence showed all the typical structural motifs of plant β-tubulins. Putative sequences for autoregulation and tubulin mRNA stability, GTP and Ca2+/MAPs (microtubule-associated proteins) binding sites were present. Southern blot analysis of chickpea genomic DNA revealed that there are multiple β-tubulin genes. The level of expression of β-tubulin genes was correlated with the rate of growth in either seedlings and adult plants. The transcript levels of β-tubulin genes were higher in actively elongating tissues such as etiolated epicotyls, roots and stem tissues of adult plants. Brassinolide-induced growth in chickpea epicotyls was accompanied by promotion of the expression of the gene coding for β-tubulin.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

References

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