Serological studies have characterized the presence of the bovine viral diarrhea virus (BVDV) infection in water buffalo herds worldwide. However, the molecular characterization of BVDV strains circulating in this animal species is uncommon. The aim of this study was to identify young water buffalo with acute infection and characterize the subgenotype of the infecting wild-type BVDV strain. Two dairy water buffalo herds from Northeastern Brazil were selected based on the results of virus neutralization test which showed high titers of anti-BVDV antibodies. To identify viremic animals, the BVDV RNA was assessed by RT-PCR assay in serum samples from 44 asymptomatic young water buffalos, where 31 serum samples from herd A and 13 from herd B. Amplicons with 288 bp of BVDV 5’UTR region were obtained in 7 (15.9%) serum samples (herd A, n =5; herd B, n = 2). One good-quality amplicon from each herd was selected for nucleotide sequencing. The phylogenetic analysis demonstrated that the two BVDV wild-type strains clustered with BVDV strains of the subgenotype 1b. This study identified for the first time the active infection by BVDV subgenotype 1b in two dairy water buffalo herds from Brazil. These results highlight the importance of that, as well
Tropical Animal Health and Production – Springer Journals
Published: May 31, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera