Bluetongue virus induces apoptosis in cultured mammalian cells by both caspase-dependent extrinsic and intrinsic apoptotic pathways

Bluetongue virus induces apoptosis in cultured mammalian cells by both caspase-dependent... Bluetongue virus (BTV) causes haemorrhagic disease in sheep and induces death in cultured mammalian cells. In the present study, BTV-induced apoptotic pathways in Vero cells were elucidated. Cells infected with BTV at 0.1 m.o.i underwent DNA fragmentation and membrane blebbing within 48 h postinfection. BTV-induced apoptosis was blocked by the pan-caspase inhibitor, z-VAD-FMK. Immuno-blotting using anti-caspase-8 and -9 antibodies detected the activation of the respective caspases. Flow cytometry analyses following 3, 3′ dihexyloxacarbocyanine iodide staining revealed the loss of mitochondrial membrane potential. Our study confirms the involvement of both caspase-dependent extrinsic and intrinsic pathways of apoptosis in BTV-infected cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Bluetongue virus induces apoptosis in cultured mammalian cells by both caspase-dependent extrinsic and intrinsic apoptotic pathways

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Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-007-0988-z
Publisher site
See Article on Publisher Site

Abstract

Bluetongue virus (BTV) causes haemorrhagic disease in sheep and induces death in cultured mammalian cells. In the present study, BTV-induced apoptotic pathways in Vero cells were elucidated. Cells infected with BTV at 0.1 m.o.i underwent DNA fragmentation and membrane blebbing within 48 h postinfection. BTV-induced apoptosis was blocked by the pan-caspase inhibitor, z-VAD-FMK. Immuno-blotting using anti-caspase-8 and -9 antibodies detected the activation of the respective caspases. Flow cytometry analyses following 3, 3′ dihexyloxacarbocyanine iodide staining revealed the loss of mitochondrial membrane potential. Our study confirms the involvement of both caspase-dependent extrinsic and intrinsic pathways of apoptosis in BTV-infected cells.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2007

References

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