Blue native electrophoresis as a tool for detection of PIP-containing protein complexes in the plasma membranes

Blue native electrophoresis as a tool for detection of PIP-containing protein complexes in the... Blue native electrophoresis (BN-PAGE) is presently considered as one of effective methods for the identification of membrane protein complexes. The choice of a nonionic detergent and the detergent to protein ratio are critically important. Our experiments with plasma membranes of etiolated pea (Pisum sativum L.) seedlings showed that various nonionic detergents—digitonin, dodecyl maltoside, and Triton X-100—solubilized similar assortments of protein complexes. Irrespective of the detergent type, PIP aquaporins were always observed in the 440-kD protein complex. Only in the case of dodecyl maltoside, the PIP aquaporins were also revealed in the complexes with the lower and higher molecular weights when the detergent/protein ratio increased. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Blue native electrophoresis as a tool for detection of PIP-containing protein complexes in the plasma membranes

Loading next page...
 
/lp/springer_journal/blue-native-electrophoresis-as-a-tool-for-detection-of-pip-containing-96ceBQu5Vx
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443713040158
Publisher site
See Article on Publisher Site

Abstract

Blue native electrophoresis (BN-PAGE) is presently considered as one of effective methods for the identification of membrane protein complexes. The choice of a nonionic detergent and the detergent to protein ratio are critically important. Our experiments with plasma membranes of etiolated pea (Pisum sativum L.) seedlings showed that various nonionic detergents—digitonin, dodecyl maltoside, and Triton X-100—solubilized similar assortments of protein complexes. Irrespective of the detergent type, PIP aquaporins were always observed in the 440-kD protein complex. Only in the case of dodecyl maltoside, the PIP aquaporins were also revealed in the complexes with the lower and higher molecular weights when the detergent/protein ratio increased.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jun 18, 2013

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from Google Scholar, PubMed
Create lists to organize your research
Export lists, citations
Access to DeepDyve database
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off