Biosensors based on modified screen-printed enzyme electrodes for monitoring of fermentation processes

Biosensors based on modified screen-printed enzyme electrodes for monitoring of fermentation... Biosensor array based on modified screen-printed electrodes containing enzymes (glucose oxidase, alcohol oxidase, lactate oxidase, mixture of glucose oxidase and γ-amylase) immobilized in a membrane of cross-linked bovine serum albumin was developed for the determination of the content of glucose, ethanol, lactic acid, and starch in fermentation media. The analytical and metrological characteristics of the developed biosensor system were determined. Samples of the fermentation mass and of alcoholic and fermented milk products were analyzed. Statistical analysis of the results obtained showed that the results of determining the content of glucose, ethanol, lactic acid, and starch with the developed biosensor system and by reference methods differed insignificantly. The developed biosensor system is considered as a base for fabricating a prototype of an analyzer for use in fermentation biotechnology. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Applied Chemistry Springer Journals

Biosensors based on modified screen-printed enzyme electrodes for monitoring of fermentation processes

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Chemistry; Chemistry/Food Science, general; Industrial Chemistry/Chemical Engineering
ISSN
1070-4272
eISSN
1608-3296
D.O.I.
10.1134/S1070427215030167
Publisher site
See Article on Publisher Site

Abstract

Biosensor array based on modified screen-printed electrodes containing enzymes (glucose oxidase, alcohol oxidase, lactate oxidase, mixture of glucose oxidase and γ-amylase) immobilized in a membrane of cross-linked bovine serum albumin was developed for the determination of the content of glucose, ethanol, lactic acid, and starch in fermentation media. The analytical and metrological characteristics of the developed biosensor system were determined. Samples of the fermentation mass and of alcoholic and fermented milk products were analyzed. Statistical analysis of the results obtained showed that the results of determining the content of glucose, ethanol, lactic acid, and starch with the developed biosensor system and by reference methods differed insignificantly. The developed biosensor system is considered as a base for fabricating a prototype of an analyzer for use in fermentation biotechnology.

Journal

Russian Journal of Applied ChemistrySpringer Journals

Published: Jun 25, 2015

References

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