Biophysical parameters as informative tools for elucidating the causes of root growth retardation under stressful conditions

Biophysical parameters as informative tools for elucidating the causes of root growth retardation... The root growth rate in barley (Hordeum vulgare L.) seedlings was measured in parallel with temporal changes in longitudinal (δl) and transverse (δD/D) cell-wall extensibilities and membrane hydraulic conductivity (L p) in the root extension zone. The root growth rate and biophysical parameters examined were sensitive to UV-B irradiation of shoots or roots and to excessive content of ammonium, glutamate, or nickel in the nutrient medium. The root responses to the above treatments were compared with the effects of abscisic acid, salicylate, hydrogen peroxide, diethylstilbestrol, α-naphthyl acetate, oryzalin, and ionomycin. The progressive reduction of root growth under the action of various stressors was accompanied by typical temporal patterns of the growth zone parameters: the δl extensibility declined monotonically, while δD/D and L p changed nonmonotonically, exhibiting the reversion from the initial decrease to the eventual increase above the control values. The decline of δl indicated that the root growth suppression was mainly due to changes in cell-wall mechanical properties caused probably by disorganization of cortical microtubules. It was found that the decline in δD/D and L p was caused primarily by the appearance of oxidative stress, disorders in cytoplasmic H+ homeostasis in root cells, and the consequent transient activation of the plasmalemmal H+-pump. Conversely, the increase in δD/D and L p upon the abrupt retardation of root growth was presumably caused by the increase in cytoplasmic Ca2+ content, disassembling of cortical microtubules, and by partial inhibition of the plasmalemmal H+-pump. The reversion of δD/D and L p changes upon progressive reduction of root growth can be used as an indicator to distinguish moderate and severe stress conditions in the root growth zone. Furthermore, this reversion indicates the increasing disbalance in the homeostasis of reactive oxygen species, cytosolic Ca2+, and cytosolic H+ upon severe stress. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Biophysical parameters as informative tools for elucidating the causes of root growth retardation under stressful conditions

Loading next page...
 
/lp/springer_journal/biophysical-parameters-as-informative-tools-for-elucidating-the-causes-s0SCToEy82
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2012 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443711060100
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial