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Bioencapsulation and Colonization Characteristics of Lactococcus lactis subsp. lactis CF4MRS in Artemia franciscana: a Biological Approach for the Control of Edwardsiellosis in Larviculture

Bioencapsulation and Colonization Characteristics of Lactococcus lactis subsp. lactis CF4MRS in... Predominance of beneficial bacteria helps to establish a healthy microbiota in fish gastrointestinal system and thus to reduce emerging pathogen. In this study, the colonization efficacy of Lactococcus lactis subsp. lactis CF4MRS in Artemia franciscana and its potential as a probiotic in suppressing Edwardsiella sp. infection were investigated in vivo. The colonization extent of the bioencapsulated L. lactis was established through visualization of gfp gene-transformed L. lactis in A. franciscana. Here, we demonstrate that when A. franciscana is administrated with L. lactis at 108 CFU mL−1 for 8 h, the highest relative percentage of survival (RPS = 50.0) is observed after inoculation with Edwardsiella sp. The total counts of L. lactis entrapped in Artemia were the highest (ranged from 3.2 to 5.1 × 108 CFU mL−1), when 108–109 CFU mL−1 of L. lactis was used as starting inoculum, with the bioencapsulation performed within 8–24 h. Fluorescent microscopy showed gfp-transformed L. lactis colonized the external trunk surfaces, mid-gut and locomotion antennules of the A. franciscana nauplii. These illustrations elucidate the efficiency of colonization of L. lactis in the gastrointestinal tract and on the body surfaces of Artemia. In conclusion, L. lactis subsp. lactis CF4MRS shows a good efficacy of colonization in Artemia and has the potential for biocontrol/probiotic activity against Edwardsiella sp. infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Marine Biotechnology Springer Journals

Bioencapsulation and Colonization Characteristics of Lactococcus lactis subsp. lactis CF4MRS in Artemia franciscana: a Biological Approach for the Control of Edwardsiellosis in Larviculture

Loh, Jiun; Kay, Gemma; Ting, Adeline
Marine Biotechnology , Volume 20 (3) – Apr 13, 2018
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10 pages

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Life Sciences; Freshwater & Marine Ecology; Microbiology; Zoology; Engineering, general
ISSN
1436-2228
eISSN
1436-2236
DOI
10.1007/s10126-018-9813-9
pmid
29654379
Publisher site
See Article on Publisher Site

Abstract

Predominance of beneficial bacteria helps to establish a healthy microbiota in fish gastrointestinal system and thus to reduce emerging pathogen. In this study, the colonization efficacy of Lactococcus lactis subsp. lactis CF4MRS in Artemia franciscana and its potential as a probiotic in suppressing Edwardsiella sp. infection were investigated in vivo. The colonization extent of the bioencapsulated L. lactis was established through visualization of gfp gene-transformed L. lactis in A. franciscana. Here, we demonstrate that when A. franciscana is administrated with L. lactis at 108 CFU mL−1 for 8 h, the highest relative percentage of survival (RPS = 50.0) is observed after inoculation with Edwardsiella sp. The total counts of L. lactis entrapped in Artemia were the highest (ranged from 3.2 to 5.1 × 108 CFU mL−1), when 108–109 CFU mL−1 of L. lactis was used as starting inoculum, with the bioencapsulation performed within 8–24 h. Fluorescent microscopy showed gfp-transformed L. lactis colonized the external trunk surfaces, mid-gut and locomotion antennules of the A. franciscana nauplii. These illustrations elucidate the efficiency of colonization of L. lactis in the gastrointestinal tract and on the body surfaces of Artemia. In conclusion, L. lactis subsp. lactis CF4MRS shows a good efficacy of colonization in Artemia and has the potential for biocontrol/probiotic activity against Edwardsiella sp. infection.

Journal

Marine Biotechnology – Springer Journals

Published: Apr 13, 2018

References

  • Potency testing of fish vaccines
    Amend, DF
  • Standard methods for the examination of water and wastewater
  • Effect of probiotics against marine pathogenic bacteria on Artemia franciscana
    Badhul Haq, MA; Vijayasanthi, P; Vignesh, R; Shalini, R; Somnath Chakraborty, R; Rajaram, R
  • An evaluation of two probiotic bacterial strains, Enterococcus faecium SF 68 and Bacillus toyoi, for reducing edwardsiellosis in cultured European eel, Anguilla anguilla L.
    Chang, CI; Liu, WY
  • A study on the bacterial flora of giant prawn, Macrobrachium rosenbergii, larvae fed with Artemia salina nauplii
    Colorni, A
  • High efficiency electroporation of Lactococcus lactis subsp. lactis LM0230
    Dornan, S; Collins, MA
  • Expression of green fluorescent protein in Lactococcus lactis
    Fernández de Palencia, P; Nieto, C; Acebo, P; Espinosa, M; López, P
  • Selection of candidate probionts by two different screening strategies from Atlantic cod (Gadus morhua L.) larvae
    Fjellheim, AJ; Klinkenberg, G; Skjermo, J; Aasen, IM; Vadstein, O
  • Encapsulation capacity of Artemia nauplii with customized probiotics for use in cultivation of western king prawns (Penaeus latisulcatus Kishinouye, 1968)
    Hai, NV; Buller, N; Fotedar, R
  • Lactobacillus sakei BK19 enriched diet enhances the immunity status and disease resistance to streptococcosis infection in kelp grouper, Epinephelus bruneus
    Harikrishnan, R; Balasundaram, C; Heo, MS
  • High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media
    Holo, H; Nes, IF
  • Selection of lactic acid bacteria as candidate probiotics and in vivo test on Artemia nauplii
    Lamari, F; Sadok, K; Bakhrouf, A; Gatesoupe, FJ
  • Isolation of putative probionts from cod rearing environment
    Lauzon, HL; Gudmundsdottir, S; Pedersen, MH; Budde, BB; Gudmundsdottir, BK
  • In vitro assessment on intestinal microflora from commonly farmed fishes for control of the fish pathogen Edwardsiella tarda
    Loh, JY; Lim, YY; Harmin, SA; Ting, ASY
  • Effects of potential probiotic Lactococcus lactis subsp. lactis on digestive enzymatic activities of live feed Artemia franciscana
    Loh, JY; Ting, ASY
  • Effect of common aquaculture chemicals against Edwardsiella ictaluri and E. tarda
    Mainous, ME; Smith, SA; Kuhn, DD
  • Influence of yeast quality on performance of gnotobiotically grown Artemia
    Marques, A; François, JM; Dhont, J; Bossier, P; Sorgeloos, P
  • Possible involvement of Artemia as live diet in the transmission of cryptosporidiosis in cultured fish
    Méndez-Hermida, F; Gómez-Couso, H; Ares-Mazás, E
  • Modulating gut microbiota and digestive enzyme activities of Artemia urmiana by administration of different levels of Bacillus subtilis and Bacillus licheniformis
    Motlagh, HRZ; Farhangi, M; Rafiee, G; Noori, F
  • Bacterial flora associated with larval rearing of the giant freshwater prawn, Macrobrachium rosenbergii
    Phatarpekar, PV; Kenkre, VD; Sreepada, RA; Desai, UM; Achuthankutty, CT
  • Novel plasmid-based genetic tools for the study of promoters and terminators in Streptococcus pneumoniae and Enterococcus faecalis
    Ruiz-Cruz, S; Solano-Collado, V; Espinosa, M; Bravo, A
  • Construction of a plasmid vector based on the pMV158 replicon for cloning and inducible gene expression in Streptococcus pneumonia
    Ruiz-Masό, JA; Lόpez-Anguilar, C; Nieto, C; Sanz, M; Burόn, P; Espinosa, M; Solar, G
  • A study of the aerobic heterotrophic bacterial flora of hatchery-reared eggs, larvae and post-larvae of Penaeus indicus
    Sahul Hameed, AS
  • Development of a bacterial challenge test for gnotobiotic Nile tilapia Oreochromis niloticus larvae
    Situmorang, ML; Dierckens, K; Mlingi, FT; Delsen, B; Bossier, P
  • Experimental exposure of Artemia to Hepatopancreatic parvo-like virus and subsequent transmission to post-larvae of Penaeus monodon
    Sivakumar, VK; Sarathi, M; Venkatesan, C; Sivaraj, A; Sahul Hameed, AS
  • Antibacterial effect of Lactococcus lactis subsp. lactis on Artemia fransciscana and Dicentrarchus labrax larvae against the fish pathogen Vibrio anguillarum
    Touraki, M; Karamanlidou, G; Koziotis, M; Christidis, I
  • Pediococcus acidilactici in the culture of turbot (Psetta maxima) larvae: administration pathways
    Villamil, L; Figueras, A; Planas, M; Novoa, B
  • The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activitiy, survival and growth in the Indian white shrimp (Fenneropenaeus indicus)
    Ziaei-Nejad, S; Rezaei, MH; Takami, GA; Lovett, DL; Mirvaghefi, AR; Shakouri, M