Based on the Prussian blue spectrophotometric method, one high-throughput screening strategy for screening lignin-degrad- ing microorganisms was built on 24-well plate at room temperature. One high activity of alkali lignin-degrading strain Rhodococcus pyridinivorans CCZU-B16 was isolated from soil. After the optimization of biodegradation, 30.2% of alkali lignin (4 g/L) was degraded under the nitrogen-limited condition (30/1 of C/N ratio; g/g) at 30 °C for 72 h. It was found that syringyl (S) units and guaiacyl (G) in lignin decreased after biodegradation. Moreover, the accumulated lipid in cells had a fatty acid profile rich in C16 and C18 with four major constituent fatty acids including palmitic acid (C16:0; 22.4%), palmitoleic acid (C16:1; 21.1%), stearic acid (C18:0; 16.2%), and oleic acid (C18:1; 23.1%). In conclusion, Rhodococcus pyridinivorans CCZU-B16 showed high potential application in future. Graphical abstract Keywords Alkali lignin · Biodegradation · High-throughput screening · Lipid · Rhodococcus pyridinivorans CCZU-B16. Introduction Lignin, the second most abundant terrestrial polymer, Electronic supplementary material The online version of this constitutes ∼ 15–30% of lignocellulosic biomass and is article (https://doi.org/10.1007/s00449-017-1884-x) contains more energy dense than cellulose and hemicellulose due supplementary material, which is available to authorized users. to its higher carbon-to-oxygen ratio [1, 2]. It is a random Extended author information available
Bioprocess and Biosystems Engineering – Springer Journals
Published: Dec 26, 2017
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