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R. Bessen, R. Marsh (1992)
Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agentJournal of Virology, 66
S. Prusiner (1982)
Novel proteinaceous infectious particles cause scrapie.Science, 216 4542
B. Oesch, M. Jensen, P. Nilsson, J. Fogh (1994)
Properties of the scrapie prion protein: quantitative analysis of protease resistance.Biochemistry, 33 19
C. Clouscard, P. Beaudry, Jean-Michel Elsen, David Milan, M. Dussaucy, C. Bounneau, François Schelcher, J. Chatelain, Jean-Marie Launay, Jean-Louis Laplanche (1995)
Different allelic effects of the codons 136 and 171 of the prion protein gene in sheep with natural scrapie.The Journal of general virology, 76 ( Pt 8)
M. McKinley, D. Bolton, S. Prusiner (1983)
A protease-resistant protein is a structural component of the Scrapie prionCell, 35
R. Bessen, D. Kocisko, G. Raymond, S. Nandan, P. Lansbury, B. Caughey (1995)
Non-genetic propagation of strain-specific properties of scrapie prion proteinNature, 375
(1976)
The pathology of natural and experimental scrapie. In: Kimberlin RH (ed) Slow virus diseases of animal and man
Piero Parchi, R. Castellani, S. Capellari, B. Ghetti, K. Young, S. Chen, M. Farlow, D. Dickson, A. Sima, J. Trojanowski, R. Petersen, P. Gambetti (1996)
Molecular basis of phenotypic variability in sporadc creudeldt‐jakob diseaseAnnals of Neurology, 39
W. Goldmann, N. Hunter, Grace Smith, James Foster, James Hope (1994)
PrP genotype and agent effects in scrapie: change in allelic interaction with different isolates of agent in sheep, a natural host of scrapie.The Journal of general virology, 75 ( Pt 5)
J. Bernillon, J. Wallach (1993)
Design of a new multiple antigen peptide system, using 9-fluorenylmethoxycarbonyl (FMOC) strategyBiotechnology Techniques, 7
W. Goldmann, Nora HUNTERt, James Fosters, J. Salbaum, Konrad Beyreuther, James Hopes (1990)
Two alleles of a neural protein gene linked to scrapie in sheep.Proceedings of the National Academy of Sciences of the United States of America, 87 7
R. Kimberlin, C. Walker, H. Fraser (1989)
The genomic identity of different strains of mouse scrapie is expressed in hamsters and preserved on reisolation in mice.The Journal of general virology, 70 ( Pt 8)
H. Büeler, A. Aguzzi, A. Sailer, R. Greiner, P. Autenried, M. Aguet, C. Weissmann (1993)
Mice devoid of PrP are resistant to scrapieCell, 73
M. Groschup, Eberhard Pfaff (1993)
Studies on a species-specific epitope in murine, ovine and bovine prion protein.The Journal of general virology, 74 ( Pt 7)
M. Beekes, Elizabeth Baldauf, Heino Diringer (1996)
Sequential appearance and accumulation of pathognomonic markers in the central nervous system of hamsters orally infected with scrapie.The Journal of general virology, 77 ( Pt 8)
R. Kimberlin, C. Walker, G. Millson, D. Taylor, P. Robertson, A. Tomlinson, A. Dickinson (1983)
Disinfection studies with two strains of mouse-passaged scrapie agent Guidelines for Creutzfeldt-Jakob and related agentsJournal of the Neurological Sciences, 59
S. Prusiner, M. Füzi, M. Scott, D. Serban, H. Serban, A. Taraboulos, J. Gabriel, G. Wells, J. Wilesmith, R. Bradley (1993)
Immunologic and molecular biologic studies of prion proteins in bovine spongiform encephalopathy.The Journal of infectious diseases, 167 3
H. Fraser (1976)
The pathology of a natural and experimental scrapie.Frontiers of biology, 44
G. Telling, Piero Parchi, S. DeArmond, P. Cortelli, P. Montagna, R. Gabizon, J. Mastrianni, E. Lugaresi, P. Gambetti, S. Prusiner (1996)
Evidence for the Conformation of the Pathologic Isoform of the Prion Protein Enciphering and Propagating Prion DiversityScience, 274
M. Bruce, A. Dickinson, H. Fraser (1976)
CEREBRAL AMYLOIDOSIS IN SCRAPIE IN THE MOUSE: EFFECT OF AGENT STRAIN AND MOUSE GENOTYPENeuropathology and Applied Neurobiology, 2
R. Carp, S. Callahan, E. Sersen, R. Moretz (1984)
Preclinical changes in weight of scrapie-infected mice as a function of scrapie agent-mouse strain combination.Intervirology, 21 2
A. Dickinson, H. Fraser (1977)
Scrapie: Pathogenesis in Inbred Mice: An Assessment of Host Control and Response Involving Many Strains of Agent
(1994)
The major species speci®c epitope in prions proteins of ruminants
J. Collinge, K. Sidle, J. Meads, J. Ironside, A. Hill (1996)
Molecular analysis of prion strain variation and the aetiology of 'new variant' CJDNature, 383
I. Zlotnik (1962)
The Pathology of Scrapie: A Comparative Study of Lesions in the Brain of Sheep and Goats
D. Westaway, V. Zuliani, C. Cooper, M. Costa, S. Neuman, A Jenny, L. Detwiler, S. Prusiner (1994)
Homozygosity for prion protein alleles encoding glutamine-171 renders sheep susceptible to natural scrapie.Genes & development, 8 8
R. Bessen, R. Marsh (1994)
Distinct PrP properties suggest the molecular basis of strain variation in transmissible mink encephalopathyJournal of Virology, 68
I. Pattison (1988)
Fifty years with scrapie: a personal reminiscenceVeterinary Record, 123
Y. Ikegami, M. Ito, H. Isomura, E. Momotani, K. Sasaki, Y. Muramatsu, N. Ishiguro, M. Shinagawa (1991)
Pre-clinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheepVeterinary Record, 128
R. Kimberlin, C. Walker (1978)
Evidence that the transmission of one source of scrapie agent to hamsters involves separation of agent strains from a mixture.The Journal of general virology, 39 3
R. Race, D. Ernst, A. Jenny, W. Taylor, D. Sutton, B. Caughey (1992)
Diagnostic implications of detection of proteinase K-resistant protein in spleen, lymph nodes, and brain of sheep.American journal of veterinary research, 53 6
R. Meyer, M. McKinley, K. Bowman, M. Braunfeld, R. Barry, S. Prusiner (1986)
Separation and properties of cellular and scrapie prion proteins.Proceedings of the National Academy of Sciences of the United States of America, 83 8
H. Hilmert, H. Diringer (1984)
A rapid and efficient method to enrich SAF-protein from scrapie brains of hamstersBioscience Reports, 4
Prions are infectious agents involved in neurodegenerative diseases, such as scrapie in sheep and goats, bovine spongiform encephalopathy (BSE) in cows and Creutzfeldt-Jakob disease (CJD) in humans. These pathogens are characterized by unusual properties, and, in particular, by their strong resistance to common procedures of disinfection used against conventional microorganisms. A major component of highly infectious fractions is a proteinase K-resistant prion protein PrPsc (PrP-res), the normal host prion protein PrPc being sensitive to PK (PrP-sen). We used a biochemical approach to further characterize PrPsc protein in natural sheep scrapie. Western blot analyses using rabbit antiserum that recognized both normal and pathologic sheep prion proteins, were undertaken to study the biochemical behaviour of PrPsc extracted from brains of sheep naturally infected with scrapie after protease digestion and under denaturing conditions. Increasing concentrations of urea (1 – 7 M) or GdnSCN (0.25 – 3 M) and different pH from 2 to 11 were tested for their effects on protease resistance of PrPsc. Alkaline pH (pH 10) and high concentrations of urea ( M) and GdnSCN ( M) greatly decreased the protease resistance of the prion protein. Identical experiments carried out on three different sheep from the same flock gave similar results. The biochemical behaviour of PrPsc under denaturing conditions and in the presence of proteinase K could thus provide a biochemical means for further characterization of different natural scrapie isolates.
Archives of Virology – Springer Journals
Published: Aug 1, 1997
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