Plant Mol Biol (2017) 94:657–667 DOI 10.1007/s11103-017-0632-6 Bacterial production and reconstitution in proteoliposomes of Solanum lycopersicum CAT2: a transporter of basic amino acids and organic cations 1 1 1 Teresa Maria Rosaria Regina · Michele Galluccio · Mariafrancesca Scalise · 1 1 Lorena Pochini · Cesare Indiveri Received: 12 November 2016 / Accepted: 1 July 2017 / Published online: 10 July 2017 © Springer Science+Business Media B.V. 2017 Abstract some of which are common for the two types of substrates. Key message The vacuolar SlCAT2 was cloned, Interestingly, reconstituted SlCAT2 showed competence for over-produced in E. coli and reconstituted in prote- acetylcholine transport, which was also inhibited by met- oliposomes.Arg, Ornithine and Lys were identified as formin. Kinetics of Arg and Ach transport were performed substrates. Unexpectedly, also the organic cationsTetra- from which Km values of 0.29 and 0.79 mM were derived, ethylammonium and Acetylcholine were transported respectively. indicating involvement of SlCAT2 insignaling. Abstract In land plants several transporters are involved Keywords Tonoplast · Acetylcholine · Metformin · in ion and metabolite flux across membranes of cells or Organic cations · Arginine · Ornithine intracellular organelles. The vacuolar amino acid trans- porter CAT2 from Solanum lycopersicum was investi- gated in this work. SlCAT2 was cloned from tomato flower Introduction cDNA, over-produced in Escherichia coli and purified by
Plant Molecular Biology – Springer Journals
Published: Jul 10, 2017
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