Bacterial phylotypes associated with the digestive tract of the sea urchin Paracentrotus lividus and the ascidian Microcosmus sp.

Bacterial phylotypes associated with the digestive tract of the sea urchin Paracentrotus lividus... We used sequencing and phylogenetic analysis of PCR-amplified 16S rRNA genes from bacteria that are associated with the esophagus/pharynx, stomach and intestine of two marine sympatric invertebrates but with different feeding mechanisms, namely the sea urchin Paracentrotus lividus (grazer) and the ascidian Microcomus sp. (suspension feeder). Amplifiable DNA was retrieved from all sections except the pharynx of the ascidian. Based on the inferred phylogeny of the retrieved sequences, the sea urchin’s esophagus is mainly characterized mostly by bacteria belonging to α-, γ-Proteobacteria and Bacteriodetes, most probably originating from the surrounding environment. The stomach revealed phylotypes that belonged to γ- and δ-Proteobacteria, Verrucomicrobia and Fusobacteria. Since the majority of their closest relatives are anaerobic species and they could be putative symbionts of the P. lividus stomach, in which anaerobic conditions also prevail. Seven out of eight phylotypes found in the sea urchin’s intestine belonged to sulfate reducing δ-Proteobacteria, and one to γ-Proteobacteria, with possible nutritional activities, i.e. degradation of complex organic compounds which is beneficial for the animal. The bacterial phylotypes of the ascidian digestive tract belonged only to the phyla of Actinobacteria and Proteobacteria. The stomach phylotypes of the ascidian were related to pathogenic bacteria possibly originating from the water column, while the intestine seemed to harbour putative symbiotic bacteria that are involved in the degradation of nitrogenous and other organic compounds, thus assisting ascidian nutrition. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Bacterial phylotypes associated with the digestive tract of the sea urchin Paracentrotus lividus and the ascidian Microcosmus sp.

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2007 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S1063074007020022
Publisher site
See Article on Publisher Site

Abstract

We used sequencing and phylogenetic analysis of PCR-amplified 16S rRNA genes from bacteria that are associated with the esophagus/pharynx, stomach and intestine of two marine sympatric invertebrates but with different feeding mechanisms, namely the sea urchin Paracentrotus lividus (grazer) and the ascidian Microcomus sp. (suspension feeder). Amplifiable DNA was retrieved from all sections except the pharynx of the ascidian. Based on the inferred phylogeny of the retrieved sequences, the sea urchin’s esophagus is mainly characterized mostly by bacteria belonging to α-, γ-Proteobacteria and Bacteriodetes, most probably originating from the surrounding environment. The stomach revealed phylotypes that belonged to γ- and δ-Proteobacteria, Verrucomicrobia and Fusobacteria. Since the majority of their closest relatives are anaerobic species and they could be putative symbionts of the P. lividus stomach, in which anaerobic conditions also prevail. Seven out of eight phylotypes found in the sea urchin’s intestine belonged to sulfate reducing δ-Proteobacteria, and one to γ-Proteobacteria, with possible nutritional activities, i.e. degradation of complex organic compounds which is beneficial for the animal. The bacterial phylotypes of the ascidian digestive tract belonged only to the phyla of Actinobacteria and Proteobacteria. The stomach phylotypes of the ascidian were related to pathogenic bacteria possibly originating from the water column, while the intestine seemed to harbour putative symbiotic bacteria that are involved in the degradation of nitrogenous and other organic compounds, thus assisting ascidian nutrition.

Journal

Russian Journal of Marine BiologySpringer Journals

Published: May 10, 2007

References

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