Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive promoter

Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive... To visualize phytohormone localization in plant tissues, transgenic plants comprising the GUS reporter gene are often used. However, until now only qualitative assessment of the hormone presence was available. In this work, we suggested the method for IAA quantification in transgenic DR5::GUS Arabidopsis thaliana L. plants by the analysis of digital images. An empirical quadratic dependence was established between the IAA concentration in medium and the level of GUS-dependent staining. Using this method, we demonstrated that, after A. thaliana root gravistimulation for 90 min, auxin lateral redistribution occurred. It resulted in the increase in the IAA concentration in the lower root part (in the elongation zone and apical meristem) by 200% on the average. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive promoter

Loading next page...
 
/lp/springer_journal/auxin-quantification-based-on-histochemical-staining-of-gus-under-the-fiX8xCi2uY
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2008 by MAIK Nauka
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443708050166
Publisher site
See Article on Publisher Site

Abstract

To visualize phytohormone localization in plant tissues, transgenic plants comprising the GUS reporter gene are often used. However, until now only qualitative assessment of the hormone presence was available. In this work, we suggested the method for IAA quantification in transgenic DR5::GUS Arabidopsis thaliana L. plants by the analysis of digital images. An empirical quadratic dependence was established between the IAA concentration in medium and the level of GUS-dependent staining. Using this method, we demonstrated that, after A. thaliana root gravistimulation for 90 min, auxin lateral redistribution occurred. It resulted in the increase in the IAA concentration in the lower root part (in the elongation zone and apical meristem) by 200% on the average.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 30, 2008

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off