Atypical status of bovine spongiform encephalopathy in Poland: a molecular typing study

Atypical status of bovine spongiform encephalopathy in Poland: a molecular typing study The aim of this study was to analyze molecular features of protease-resistant prion protein (PrP res ) in Western blots of BSE cases diagnosed in Poland with respect to a possible atypical status. Confirmed cases were analyzed by Western blotting with several monoclonal antibodies directed at N-terminal and core epitopes of prion protein (PrP). Most cases showed the classical glycoprofile characterized by the dominance of the di- over the monoglycosylated PrP res band, yielding di-/mono- ratios well above 2 and by reactivity with antibodies having their epitopes in bovine PrP region 110–242 (C-type cases). Surprisingly, seven cases of BSE were atypical. Six were classified as L-type based on a slightly lower molecular mass (M r ) of the non- glycosylated band with respect to C-types and a conspicuously low di-/mono- ratio of glycosylated PrP res bands approaching unity. One case was classified as H-type because of a higher M r of PrP res bands on the blot when compared with C-type cases. A characteristic epitope of H-type PrP res occurred in the 101–110 region of PrP for which only antibody 12B2 had a sufficient affinity. The occurrence of atypical cases only in animals 9 years of age and older raises questions about the mechanisms of prion diseases and the origin of BSE. Archives of Virology Springer Journals

Atypical status of bovine spongiform encephalopathy in Poland: a molecular typing study

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Copyright © 2008 by Springer-Verlag
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
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