Access the full text.
Sign up today, get DeepDyve free for 14 days.
T. Colonna, L. Huynh, D. Fambrough (1997)
Subunit Interactions in the Na,K-ATPase Explored with the Yeast Two-hybrid System*The Journal of Biological Chemistry, 272
K. Sweadner, C. Donnet (2001)
Structural similarities of Na,K-ATPase and SERCA, the Ca(2+)-ATPase of the sarcoplasmic reticulum.The Biochemical journal, 356 Pt 3
W. Kühlbrandt (2004)
Biology, structure and mechanism of P-type ATPasesNature Reviews Molecular Cell Biology, 5
A. Therien, Helen Pu, S. Karlish, R. Blostein (2001)
Molecular and Functional Studies of the Gamma Subunit of the Sodium PumpJournal of Bioenergetics and Biomembranes, 33
C. Toyoshima, H. Nomura (2002)
Structural changes in the calcium pump accompanying the dissociation of calciumNature, 418
M. Hilge, G. Siegal, G. Vuister, P. Güntert, S. Gloor, J. Abrahams (2003)
ATP-induced conformational changes of the nucleotide-binding domain of Na,K-ATPaseNature Structural Biology, 10
W. Wriggers, W. Wriggers, R. Milligan, J. McCammon (1999)
Situs: A package for docking crystal structures into low-resolution maps from electron microscopy.Journal of structural biology, 125 2-3
Collaborative Computational (1994)
The CCP4 suite: programs for protein crystallography.Acta crystallographica. Section D, Biological crystallography, 50 Pt 5
L. Cantley, L. Cantley, L. Josephson (1978)
A characterization of vanadate interactions with the (Na,K)-ATPase. Mechanistic and regulatory implications.The Journal of biological chemistry, 253 20
A. Ivanov, N. Modyanov, A. Askari (2002)
Role of the self-association of beta subunits in the oligomeric structure of Na+/K+-ATPase.The Biochemical journal, 364 Pt 1
H. Hebert, P. Purhonen, H. Vorum, K. Thomsen, A. Maunsbach (2001)
Three-dimensional structure of renal Na,K-ATPase from cryo-electron microscopy of two-dimensional crystals.Journal of molecular biology, 314 3
William Rice, Howard Young, Dwight Martin, John Sachs, David Stokes (2001)
Structure of Na+,K+-ATPase at 11-A resolution: comparison with Ca2+-ATPase in E1 and E2 states.Biophysical journal, 80 5
Helen Pu, R. Scanzano, R. Blostein (2002)
Distinct Regulatory Effects of the Na,K-ATPase γ Subunit*The Journal of Biological Chemistry, 277
L.C. Cantley, L.G. Cantley, L. Josephson (1978)
A characterization of vanadate interactions with the (Na,K)-ATPaseJ. Biol. Chem., 253
T. Jones, J. Zou, S. Cowan, M. Kjeldgaard, Serge HAGgGE (1991)
Improved methods for building protein models in electron density maps and the location of errors in these models.Acta crystallographica. Section A, Foundations of crystallography, 47 ( Pt 2)
K. Obara, N. Miyashita, Cheng Xu, I. Toyoshima, Y. Sugita, G. Inesi, C. Toyoshima (2005)
Structural role of countertransport revealed in Ca(2+) pump crystal structure in the absence of Ca(2+).Proceedings of the National Academy of Sciences of the United States of America, 102 41
C. Donnet, E. Arystarkhova, K. Sweadner (2001)
Thermal Denaturation of the Na,K-ATPase Provides Evidence for α-α Oligomeric Interaction and γ Subunit Association with the C-terminal Domain*The Journal of Biological Chemistry, 276
C. Toyoshima, H. Nomura, T. Tsuda (2004)
Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analoguesNature, 432
I. Lubarski, K. Pihakaski-Maunsbach, S. Karlish, A. Maunsbach, H. Garty (2005)
Interaction with the Na,K-ATPase and Tissue Distribution of FXYD5 (Related to Ion Channel)*Journal of Biological Chemistry, 280
C. Franzin, Jungyuen Choi, D. Zhai, John Reed, F. Marassi (2004)
Structural studies of apoptosis and ion transport regulatory proteins in membranesMagnetic Resonance in Chemistry, 42
K. Sweadner, Efren Rael, Efren Rael (2000)
The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression.Genomics, 68 1
R. Crowther, R. Henderson, Judith Smith (1996)
MRC image processing programs.Journal of structural biology, 116 1
D. Stokes, A. Pomfret, W. Rice, J. Glaves, H. Young (2006)
Interactions between Ca2+-ATPase and the pentameric form of phospholamban in two-dimensional co-crystals.Biophysical journal, 90 11
K. Obara, N. Miyashita, Cheng Xu, I. Toyoshima, Y. Sugita, G. Inesi, C. Toyoshima (2005)
Inaugural Article: Structural role of countertransport revealed in Ca2+ pump crystal structure in the absence of Ca2+., 102
C. Toyoshima, M. Nakasako, H. Nomura, H. Ogawa (2000)
Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 Å resolutionNature, 405
A. Zouzoulas, R. Blostein (2006)
Regions of the Catalytic α Subunit of Na,K-ATPase Important for Functional Interactions with FXYD 2*Journal of Biological Chemistry, 281
U. Hasler, G. Crambert, J. Horisberger, K. Geering (2001)
Structural and Functional Features of the Transmembrane Domain of the Na,K-ATPase β Subunit Revealed by Tryptophan Scanning*The Journal of Biological Chemistry, 276
K. Håkansson (2003)
The crystallographic structure of Na,K-ATPase N-domain at 2.6A resolution.Journal of molecular biology, 332 5
P. Jørgensen, K. Håkansson, S. Karlish (2003)
Structure and mechanism of Na,K-ATPase: functional sites and their interactions.Annual review of physiology, 65
E. Or, R. Goldshleger, S. Karlish (1999)
Characterization of Disulfide Cross-links between Fragments of Proteolyzed Na,K-ATPaseThe Journal of Biological Chemistry, 274
Maria Füzesi, K. Gottschalk, Moshit Lindzen, A. Shainskaya, B. Küster, H. Garty, S. Karlish (2005)
Covalent Cross-links between the γ Subunit (FXYD2) and α and β Subunits of Na,K-ATPaseJournal of Biological Chemistry, 280
Ciming Li, A. Grosdidier, G. Crambert, J. Horisberger, O. Michielin, K. Geering (2004)
Structural and Functional Interaction Sites between Na,K-ATPase and FXYD Proteins*Journal of Biological Chemistry, 279
A. Sali, T. Blundell (1993)
Comparative protein modelling by satisfaction of spatial restraints.Journal of molecular biology, 234 3
C. Toyoshima, M. Asahi, Y. Sugita, Reena Khanna, T. Tsuda, D. Maclennan (2003)
Modeling of the inhibitory interaction of phospholamban with the Ca2+ ATPaseProceedings of the National Academy of Sciences of the United States of America, 100
T. Sørensen, J. Møller, P. Nissen (2004)
Phosphoryl Transfer and Calcium Ion Occlusion in the Calcium PumpScience, 304
H. Ogawa, C. Toyoshima (2002)
Homology modeling of the cation binding sites of Na+K+-ATPaseProceedings of the National Academy of Sciences of the United States of America, 99
E. Pettersen, Thomas Goddard, Conrad Huang, Gregory Couch, Daniel Greenblatt, E. Meng, T. Ferrin (2004)
UCSF Chimera—A visualization system for exploratory research and analysisJournal of Computational Chemistry, 25
C. Toyoshima, T. Mizutani (2004)
Crystal structure of the calcium pump with a bound ATP analogueNature, 430
B. Forbush, J. Kaplan, J. Hoffman (1978)
Characterization of a new photoaffinity derivative of ouabain: labeling of the large polypeptide and of a proteolipid component of the Na, K-ATPase.Biochemistry, 17 17
Shyang-Guang Wang, R. Farley (1998)
Valine 904, Tyrosine 898, and Cysteine 908 in Na,K-ATPase α Subunits Are Important for Assembly with β Subunits*The Journal of Biological Chemistry, 273
K. Axelsen, M. Palmgren (1998)
Evolution of Substrate Specificities in the P-Type ATPase SuperfamilyJournal of Molecular Evolution, 46
Claus Olesen, T. Sørensen, R. Nielsen, J. Møller, P. Nissen (2004)
Dephosphorylation of the Calcium Pump Coupled to Counterion OcclusionScience, 306
A. Ivanov, H. Zhao, N. Modyanov (2000)
Packing of the transmembrane helices of Na,K-ATPase: direct contact between beta-subunit and H8 segment of alpha-subunit revealed by oxidative cross-linking.Biochemistry, 39 32
A. Jensen, T. Sørensen, Claus Olesen, J. Møller, P. Nissen (2006)
Modulatory and catalytic modes of ATP binding by the calcium pumpThe EMBO Journal, 25
Edmund Kunji, S. Gronau, D. Oesterhelt, R. Henderson (2000)
The three-dimensional structure of halorhodopsin to 5 A by electron crystallography: A new unbending procedure for two-dimensional crystals by using a global reference structure.Proceedings of the National Academy of Sciences of the United States of America, 97 9
P. Jørgensen (1988)
Purification of Na+,K+-ATPase: enzyme sources, preparative problems, and preparation from mammalian kidney.Methods in enzymology, 156
K. Geering (2001)
The Functional Role of β Subunits in Oligomeric P-Type ATPasesJournal of Bioenergetics and Biomembranes, 33
S. Keenan, R. DeLisle, W. Welsh, S. Paula, W. Ball (2005)
Elucidation of the Na+, K+-ATPase digitalis binding site.Journal of molecular graphics & modelling, 23 6
Na,K-ATPase transports Na+ and K+ across cell membranes and consists of α- and β-subunits. Na,K-ATPase also associates with small FXYD proteins that regulate the activity of the pump. We have used cryoelectron microscopy of two-dimensional crystals including data to 8 Å resolution to determine the three-dimensional (3-D) structure of renal Na,K-ATPase containing FXYD2, the γ-subunit. A homology model for the α-subunit was calculated from a Ca2+-ATPase structure and used to locate the additional β- and γ-subunits present in the 3-D map of Na,K-ATPase. Based on the 3-D map, the β-subunit is located close to transmembrane helices M8 and M10 and the γ-subunit is adjacent to helices M2 and M9 of the α-subunit.
The Journal of Membrane Biology – Springer Journals
Published: Jun 6, 2007
Access the full text.
Sign up today, get DeepDyve free for 14 days.