Assignment of the porcine nuclear factor I/CTF (NFI/CTF) gene to Chromosome 2q12-13 by FISH

Assignment of the porcine nuclear factor I/CTF (NFI/CTF) gene to Chromosome 2q12-13 by FISH 456 Mammalian Genome 8, Brief Data Reports 10. Szpirer C, Szpirer J, Klinga-Levan K, Stahl F, Levan G (1996) Folia as a single block in artiodactyl genomes [8]. The mapping of the Biol (Prague) 42, 175-226 porcine NFI/CTF gene to SSC2q 12-13 corroborates the conserved 11. Imai K (1996) Mamm Genome 6(Suppl), S170-S189 synteny between HSA19p and the homologous segment at SSC2cen-q21, which has also been demonstrated by a recent for- ward and reverse Zoo-FISH approach [9]. Assignment of the porcine nuclear factor Acknowledgments: We thank C. Seisenberger and E.L. Winnacker, Inst. I/CTF (NFI/CTF) gene to Chromosome fiir Biochemie LMU, Mtinchen, Germany, for kindly providing the pig NFI/CTF EMBL phage. 2q12-13 by FISH References 1. Meisteremst M, Rogge L, Donath C, Gander I, Lottspeich F, Mertz R, Lutz Friinicke, Harry Scherthan Dobner T, F6ckler R, Stelzer G, Winnacker EL (1988) FEBS Let 236, 27-32 Abteilung ftir Humanbiologie und Humangenetik, Universit~it 2. Quian F, Kruse U, Lichter P, Sippel AE (1995) Genomics 28, 66-73 Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany 3. Scherthan H, Seisenberger C, Greulich K, Winnacker EL (1994) Ge- nomics 22, 247-249 Received: 15 December 1996 / Accepted: 17 February 1997 4. Seisenberger C, Winnacker E-L, Scherthan H (1993) Hum Genet 91, Species: Pig (Sus scrofa domestica) 535-537 5. Meisterernst M, Rogge L, Foeckler R, Karaghiosoff M, Winnacker E-L Locus name: Nuclear Factor I/CTF (1989) Biochemistry 28, 8191-8200 Locus symbol: NFI/CTF 6. Rettenberger G, Klett Ch, Zechner U, Kunz J, Vogel W, Hameister H Map position: 2q12-13 (1995) Genomics 26, 372-378 Method of mapping: Fluorescence in situ hybridization (FISH) 7. Fr6nicke L, Chowdhary BP, Scherthan H, Gustavsson I (1996) Mamm Molecular reagents: An EMBL3A phage containing 40 kb of pig Genome 7, 285-290 NF1/CTF gene [1] was biotinylated and detected by avidin-FITC. 8. Mohrenweiser A, Olsen A, Archibald A, Beattie C, Burmeister M, Previously identified homologs: NFIC maps to human Chromo- Lamerdin J, Lennon G, Stewart E, Stubbs L, Weber JL, Johnson K some (Chr) 19p13 [2]. (1996) Cytogenet Cell Genet 74, 161-186 Discussion: Nuclear factor NFI/CTF is a member of a family of 9. Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat G, dimeric DNA-binding proteins. Its recognition sequence [TGG(C/ Gellin J (1996) Genomics 36, 252-262 A)Ns] is present in a number of DNA viruses and in mammalian and avian genomes [see 3]. The proteins are involved in cellular Genetic mapping of the gene encoding transcription regulation and adenovirus transcription initiation. In cysteine string protein the human genome, NFI-A,B,C and NFI-X genes have been iden- tified and mapped [2,4]. NFI-C and NFI-X are located in close Hemin Chin, 1 Myung-Soo Lyu, 20h-Joo Kwon, 1'3 proximity on human Chr 19p 13, whereas NFI-A and -B genes map to lp31.2-31.3 and 9p24.1, respectively [2]. In the mouse, Nfix has Christine A. Kozak z been assigned to 8C1-2 [7]. Of all mammalian nuclear factor I ~Laboratory of Neurochemistry, National Institute of Neurological genes isolated to date, the genomic structure of the porcine gene Disorders and Strokes, Building 36, Room 4D-20, 36 Convent Drive, has been characterized in most detail [5]. Gross regional homolo- MCS4130, Bethesda, Maryland 2089-4130, USA gies between human and porcine genomes have recently been de- 2Laboratory of Molecular Microbiology, National Institute of Allergy marcated by Zoo-FISH [6,q]. These studies revealed the presence and Infectious Diseases, National Institutes of Health, of two conserved syntenic segments between HSA19 and Bethesda Maryland 20892, USA SSC2cen-q21 and SSC6cen-q21 [7]. Comparative gene mapping 3Department of Biochemistry, Catholic University Medical College, data suggest that the p and q arms of HSA 19 are each conserved Seoul, Korea Correspondence to: L. Fr6nicke Correspondence to: H. Chin Fig. 1. (a) FISH with the porcine NFI/CTF EMBL3A phage clone to pig metaphase chromosomes. Signal doublets are revealed at both Chrs 2, namely, 2q12-13 (arrowheads). (b) Same metaphase after DAPI banding and color inversion. Mammalian Genome Springer Journals

Assignment of the porcine nuclear factor I/CTF (NFI/CTF) gene to Chromosome 2q12-13 by FISH

1 page

Loading next page...
1 Page
Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial