Assignment of the gene for outer dense fiber of sperm tails (ODF) to porcine chromosome 4p11-p14

Assignment of the gene for outer dense fiber of sperm tails (ODF) to porcine chromosome 4p11-p14 628 Mammalian Genome 8, Brief Data Reports Assignment of the gene for outer dense fiber of sperm tails (ODF) to porcine Chromosome 4pll-p14 Petra Musilovd, I~ Antonin Stratil, 2 MiluSe Jurdkovd, I Ji~i Rube~, I Stanislav Cepica 2 1Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic 2Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 277 21 Lib~chov, Czech Republic Received: 13 October 1996 / Accepted: 17 March 1997 Species: Pig (Sus scrofa domestica) Fig. 1. In situ hybridization of ODF to porcine Chr 4. The distribution of Locus name: Outer dense fiber of sperm tails silver grains representing hybridization events is displayed above the G- Locus symbol: ODF banded idiogram of pig Chr 4. Of the 967 silver grains overlying chromo- Map position: Chr 4pl 1-p14, with a major peak on bands p12-p13 somes in 155 metaphases, 130 (13.4%) grains were on Chr 4, with 86 (Fig. 1). (66.2%) of these in the peak over region 4pll-p14. As porcine Chr 4 represents 5.6% of the pig genome, this was highly significant (P < 0.001). Method of mapping: Radioactive in situ hybridization: G banding [1]. Database deposit information: EMBL Gen Bank accession num- Acknowledgments: We are indebted to Dr. I.M. Adham (Institut fur ber X69513. Humangenetik, Zentrum fur Hygiene und Humangeuetik der Universit~it, Molecular reagents: The probe was linearized plasmid pUC18 G6ttingen, Germany) for providing us with the plasmid containing the containing 530 bp EcoRI fragment of eDNA of porcine sperm porcine ODF cDNA. The study was supported by grants 514/94/1051 outer dense fiber gene [2]. (Grant Agency of the Czech Republic) and EP0960006093 (Grant Agency Previously identified homologs: Mapped homologs are in Dro- of Ministry of Agriculture of the Czech Republic). formerly designated as mst(3)gl-9 [2--4], sophila melanogaster References listed in FlyBase as Mst87F, rat rst5/1 on Chr 7 [5], cattle ODF on 1. Lahbib-Mansais Y, Mellink C, Yerle M, Gellin J (1994) Cytogenet Chr 14q23-q24 [6] and human ODF1 on Chr 8q22 [7]. Cell Genet 67, 120-125 Discussion: The accessory or outer dense fibers (ODFs) are a 2. Kim Y, Adham IM, Haack T, Kremling H, Engel W (1995) Biol Chem major component of the mammalian sperm tail. ODFs have been Hoppe-Seyler 376, 431-435 found in many groups within the animal kingdom, including in- 3. Schaffer U (1986) Mol Gen Genet 202, 219-225 sects [8]. Isolated ODFs of mammalian sperm consist of several 4. Kubn R, Sch/ifer U, Sch~ifer M (1988) EMBO J 7, 447-454 proteins. For one of these proteins, cDNAs and genomic sequences 5. Burfeind P, Belgardt B, Szpirer C, Hoyer-Fender S (1993) Eur J Bio- of rat [5], human [7], and cDNAs of pig and cattle [2] were cloned. chem 216, 497-505 The ODF protein deduced from the porcine eDNA shares 93%, 6. Friedel R, Adham IM, Roltmann OJ (1994) Mamm Genome 5, 833- 88%, and 98% sequence identity with proteins deduced from hu- 7. Gastamann O, Burfeind P,,GiJnther E, Hameister H, Spirer C, Hoyer- man, rat, and bovine cDNAs, respectively [2]. Functions of ODFs Fender S (1993) Mol Reprod Dev 36, 407-418 are not defined. It is suggested that they may help to maintain the 8. Bacceti B, Pallini V, Burrini AG (1973) J Submicrosc Cytol 5, 237- passive elastic structure and elastic recoil of the sperm tail and/or protect the sperm tail against shearing during epididymal transport 9. Baltz JM, Williams PO, Cone RA (1990) Biol Reprod 43, 485-491 [9]. The assignment of ODF to pig Chr 4p reported here is con- 10. Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H sistent with the ZOO-FISH data showing conserved synteny be- (1995) Genomics 26, 372-378 tween human Chr 8 and this pig Chr [10-12]. 11. Fr6nicke L, Chowdhary BP, Scherthan H, Gustavsson I (1996) Mamm Genome 7, 285-290 12. Goureau A, Yerle M, Schrnitz A, Riquet J, Milan D, Pinton P, Frelat Correspondence to: S. Cepica G, Gellin J (1996) Genomics 36, 252-262 Mammalian Genome Springer Journals

Assignment of the gene for outer dense fiber of sperm tails (ODF) to porcine chromosome 4p11-p14

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Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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