Mammalian Genome 9, 913–914 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 Assignment of five porcine genes by pig–mink cell hybrids to pig chromosomes 2, 5, 8, 12 1 1 1 2 2 Irina V. Koroleva, Sergey N. Malchenko, Naseer M. Shukri, Elena V. Ivanova, Sergey B. Kuznetsov, 2 1 Natalia S. Zhdanova, Christian Bendixen Department of Breeding and Genetics, Danish Institute of Agricultural Sciences, DK-8830 Tjele, Denmark Institute of Cytology and Genetics, Russian Academy of Science, Siberian Department, 630090 Novosibirsk, Russia Received: 13 November 1997 / Accepted: 22 May 1998 Comparative gene mapping makes it possible to identify homolo- for 20 s, and a final cycle of 72°C for 10 min. Seven microliter of gous chromosome segments in distantly related mammals and to the PCR products were run on 2% agarose gel in 1 × TBE after analyze genome organization and evolution (O’Brien et al. 1993). PCR reaction or after digestion with restriction enzyme Sau3A for An important aspect of such information is to allow the exchange FGFR4, MspI for MLR, and RsaI for EV12A. of information between species. Expressed genes are suitable land- marks for comparative mapping. Lyons and coworkers (1997) de- DNA sequencing and
Mammalian Genome – Springer Journals
Published: Nov 1, 1998
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