Assessment of Triazole Growth-Retarding Activity in an α-Amylase Bioassay Using Spring Barley Endosperm

Assessment of Triazole Growth-Retarding Activity in an α-Amylase Bioassay Using Spring Barley... The possibility to assess the growth-retarding activity of triazole compounds, such as paclobutrazole, uniconazole, and azovite, by an express bioassay based on the evaluation of the α-amylase activity, which hydrolyzes starch into soluble sugars in the aleurone layer of barley (Hordeum vulgare L.), was investigated. The retarding effects of triazoles, when evaluated by the inhibition of amylase activity, decreased in the following order: uniconazole > azovite > paclobutrazole ≈ chlorocholine chloride. Among barley cultivars, cv. No-sovskii 9 was the most sensitive to the tested triazole compounds. At the concentrations of 10–7 to 10–3 M, these chemicals manifested notable growth-retarding activity in the bioassays with the seedlings of long stemmed pea cv. Torsdag and spring barley, cvs. Nosovskii 9, Moskovskii 2, and Zazerskii 85. All these express bioassays were validated for assessing the triazole growth-retarding activity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Assessment of Triazole Growth-Retarding Activity in an α-Amylase Bioassay Using Spring Barley Endosperm

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Publisher
Springer Journals
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/B:RUPP.0000035753.41438.d8
Publisher site
See Article on Publisher Site

Abstract

The possibility to assess the growth-retarding activity of triazole compounds, such as paclobutrazole, uniconazole, and azovite, by an express bioassay based on the evaluation of the α-amylase activity, which hydrolyzes starch into soluble sugars in the aleurone layer of barley (Hordeum vulgare L.), was investigated. The retarding effects of triazoles, when evaluated by the inhibition of amylase activity, decreased in the following order: uniconazole > azovite > paclobutrazole ≈ chlorocholine chloride. Among barley cultivars, cv. No-sovskii 9 was the most sensitive to the tested triazole compounds. At the concentrations of 10–7 to 10–3 M, these chemicals manifested notable growth-retarding activity in the bioassays with the seedlings of long stemmed pea cv. Torsdag and spring barley, cvs. Nosovskii 9, Moskovskii 2, and Zazerskii 85. All these express bioassays were validated for assessing the triazole growth-retarding activity.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Sep 26, 2004

References

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