Assessment of Population Differentiation Using DNA Fingerprinting and Modified Wright's FST-Statistics

Assessment of Population Differentiation Using DNA Fingerprinting and Modified Wright's... Using our results and literature data on multilocus DNA fingerprinting, we propose a method of obtaining unbiased estimates of the between-population genetic similarity index and a measure of population subdivision based on modified Wright's F ST-statistics. On the basis of multiple comparison T 2 Hotelling's test and Holmes' procedure, the F ST-statistics was applied to assess differentiation of four (Pacific and Atlantic) subpopulations of humpback whale Megaptera novaeangliae, six populations of Californian island gray fox Urocyon littoralis, and geographically isolated Ob' and Yakutia populations of Siberian white crane Crus leucogeranus. It was shown that the regional humpback whale subpopulations do not constitute a single panmictic unit (P < 10–4). The subdivision index of the Pacific and Atlantic populations expressed in terms of F-statistics varied from 0.101 to 0.157. The differentiation estimates for the island fox populations, which ranged from 0.2109 to 0.4027, indicate that subdivision of these populations is a function of the distance between the islands, island size, and population size. In particular, the smallest and the greatest differences were found respectively between the populations of the geographically closest northern islands (F ST = 0.2157, F ST = 0.2109) and between those of the most distant northern and southern islands (F ST = 0.4027, F ST = 0.3869). Subdivision of the island populations with minimum areas and low population number was intermediate (F ST = 0.3789). Mean values of heterozygosity, within-population genetic similarity index, and the number of coinciding fragments for two random individuals of Siberian white crane from the Ob' and Yakutia population were not statistically significantly different (P ≥ 0.852, P ≥ 0.491, P ≥ 0.325). However, pairwise comparisons of mean F ST values indicated that the differentiation estimates for samples from these populations fall within the limits of population subdivision (P = 0.01). The subdivision estimate (0.108–0.133) of various groups of Siberian white cranes is comparable to interregional subdivision of humpback whale. Based on the results of this study, we recommend the approach based on modified Wright's F ST-statistics for studying genetic population structure aimed at detecting population subdivision. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Assessment of Population Differentiation Using DNA Fingerprinting and Modified Wright's FST-Statistics

Loading next page...
 
/lp/springer_journal/assessment-of-population-differentiation-using-dna-fingerprinting-and-mRfA3brqte
Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1022479725574
Publisher site
See Article on Publisher Site

Abstract

Using our results and literature data on multilocus DNA fingerprinting, we propose a method of obtaining unbiased estimates of the between-population genetic similarity index and a measure of population subdivision based on modified Wright's F ST-statistics. On the basis of multiple comparison T 2 Hotelling's test and Holmes' procedure, the F ST-statistics was applied to assess differentiation of four (Pacific and Atlantic) subpopulations of humpback whale Megaptera novaeangliae, six populations of Californian island gray fox Urocyon littoralis, and geographically isolated Ob' and Yakutia populations of Siberian white crane Crus leucogeranus. It was shown that the regional humpback whale subpopulations do not constitute a single panmictic unit (P < 10–4). The subdivision index of the Pacific and Atlantic populations expressed in terms of F-statistics varied from 0.101 to 0.157. The differentiation estimates for the island fox populations, which ranged from 0.2109 to 0.4027, indicate that subdivision of these populations is a function of the distance between the islands, island size, and population size. In particular, the smallest and the greatest differences were found respectively between the populations of the geographically closest northern islands (F ST = 0.2157, F ST = 0.2109) and between those of the most distant northern and southern islands (F ST = 0.4027, F ST = 0.3869). Subdivision of the island populations with minimum areas and low population number was intermediate (F ST = 0.3789). Mean values of heterozygosity, within-population genetic similarity index, and the number of coinciding fragments for two random individuals of Siberian white crane from the Ob' and Yakutia population were not statistically significantly different (P ≥ 0.852, P ≥ 0.491, P ≥ 0.325). However, pairwise comparisons of mean F ST values indicated that the differentiation estimates for samples from these populations fall within the limits of population subdivision (P = 0.01). The subdivision estimate (0.108–0.133) of various groups of Siberian white cranes is comparable to interregional subdivision of humpback whale. Based on the results of this study, we recommend the approach based on modified Wright's F ST-statistics for studying genetic population structure aimed at detecting population subdivision.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 7, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off