Assessment of neurohepatic DNA damage in male Sprague–Dawley rats exposed to organophosphates and pyrethroid insecticides

Assessment of neurohepatic DNA damage in male Sprague–Dawley rats exposed to organophosphates... The current work was undertaken to test the genotoxic potential of chlorpyrifos (CPF), dimethoate, and lambda cyhalothrin (LCT) insecticides in rat brain and liver using the single cell gel electrophoresis (comet assay). Three groups of adult male Sprague–Dawley rats were exposed orally to one third LD50of CPF, dimethoate, or LCT for 24 and 48 h while the control group received corn oil. Serum samples were collected for estimation of malondialdehyde (MDA) and glutathione peroxidase (GPx); the brain and liver samples were used for comet assay and for histopathological examination. Results showed that signs of neurotoxicity appeared clinically as backward stretching of hind limb and splayed gait in dimethoate and LCT groups, respectively. CPF, LCT, and dimethoate induced oxidative stress indicated by increased MDA and decreased GPx levels. CPF and LCT caused severe DNA damage in the brain and liver at 24 and 48 h indicated by increased percentage of DNA in tail, tail length, tail moment, and olive tail moment. Dimethoate induced mild DNA damage in the brain and liver at 48 h. Histopathological changes were observed in the cerebrum, cerebellum, and liver of exposed rats. The results concluded that CPF, LCT, and dimethoate insecticides induced oxidative stress and DNA damage associated with histological changes in the brain and liver of exposed rats. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Science and Pollution Research Springer Journals

Assessment of neurohepatic DNA damage in male Sprague–Dawley rats exposed to organophosphates and pyrethroid insecticides

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Environment; Environment, general; Environmental Chemistry; Ecotoxicology; Environmental Health; Atmospheric Protection/Air Quality Control/Air Pollution; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution
ISSN
0944-1344
eISSN
1614-7499
DOI
10.1007/s11356-018-1776-x
Publisher site
See Article on Publisher Site

Abstract

The current work was undertaken to test the genotoxic potential of chlorpyrifos (CPF), dimethoate, and lambda cyhalothrin (LCT) insecticides in rat brain and liver using the single cell gel electrophoresis (comet assay). Three groups of adult male Sprague–Dawley rats were exposed orally to one third LD50of CPF, dimethoate, or LCT for 24 and 48 h while the control group received corn oil. Serum samples were collected for estimation of malondialdehyde (MDA) and glutathione peroxidase (GPx); the brain and liver samples were used for comet assay and for histopathological examination. Results showed that signs of neurotoxicity appeared clinically as backward stretching of hind limb and splayed gait in dimethoate and LCT groups, respectively. CPF, LCT, and dimethoate induced oxidative stress indicated by increased MDA and decreased GPx levels. CPF and LCT caused severe DNA damage in the brain and liver at 24 and 48 h indicated by increased percentage of DNA in tail, tail length, tail moment, and olive tail moment. Dimethoate induced mild DNA damage in the brain and liver at 48 h. Histopathological changes were observed in the cerebrum, cerebellum, and liver of exposed rats. The results concluded that CPF, LCT, and dimethoate insecticides induced oxidative stress and DNA damage associated with histological changes in the brain and liver of exposed rats.

Journal

Environmental Science and Pollution Research – Springer Journals

Published: Mar 23, 2018

References

  • Effect of Solanum nigrum Linn against lambda cyhalothrin-induced toxicity in rats
    Abd Elkawy, M; Oliman, G; Abd-El Rehim, E
  • Glutathione peroxidase and glucose 6 phosphate dehydrogenase activities in bovine blood and liver
    Abd Ellah, MR; Nishimori, K; Goryo, M; Okada, K; Yasuda, J
  • Caffeic acid and quercetin protect erythrocytes against the oxidative stress and the genotoxic effects of lambda-cyhalothrin in vitro
    Abdallah, F; Fetoui, H; Fakhfakh, F; Keskes, L
  • Toxicological influences of lambda cyhalothrin and evaluation of the toxicity ameliorative effect of pomegranate in albino rats, global
    Abdel-Mobdy, YE; Abdel-Rahim, EA
  • Dimethoate-induced oxidative stress and morphological changes in the liver of Guinea pig and the protective effect of vitamin C and E
    Al-Awthan, YS; Mohamed, A; Gamal, HE; Esam, MA
  • Oxidative stress and genetic damage among workers exposed primarily to organophosphate and pyrethroid pesticides
    Arce, ZR; Rojas-García, AE; Benitez-Trinidad, A; Herrera-Moreno, JF; Medina-Díaz, IM; Barrón-Vivanco, BS; Villegas, GP; Hernández-Ochoa, I; Sólis Heredia, MJ; Bernal-Hernández, YY
  • Effect of pesticides on cell survival in liver and brain rat tissues
    Astiz, M; Alaniz, MJ; Marra, CA
  • Comparative effects of TCDD, endrin, naphthalene and chromium (IV) on oxidative stress and tissue damage in the liver and brain tissues of mice
    Bagchi, D; Balmoori, J; Bagchi, M; Ye, X; Williams, CB; Stohs, SJ
  • Endosulfan induces changes in spontaneous swimming activity and acetylcholinesterase activity of Jenynsia multidentata (Anablepidae, Cyprinodontiformes)
    Ballesteros, ML; Durando, PE; Nores, ML; Diaz, MP; Bistoni, MA; Wunderlin, DA
  • Exposure to low doses of endosulfan and chlorpyrifos modifies endogenous antioxidants in tissue of rats
    Bebe, FN; Panemangalore, M
  • Cellular responses to reactive oxygen species induced DNA damage and aging
    Bertram, C; Hass, R
  • Genotoxicity of pesticides: a review of human biomonitoring studies
    Bolognesi, C
  • Activity of organophosphorus insecticides in bacterial tests for mutagenicity and DNA repair-direct alkylation vs metabolic activation and breakdown. I: butanoate, vinylbutonate, dichlorvos, demethyldichlorovos and demethyl vinylbutonate
    Braun, R; Schoneich, J; Weissflog, L; Debek, W
  • Cytogenitic effects of lambda-cyhalothrin on wistar rat bone marrow
    Celik, A; Mazmanci, BC; Amlica, Y; Askin, AC; Omelekoglu, U
  • Evaluation of cytogenetic effects of lambda-cyhalothrin on Wistar rat bone marrow by gavage administration
    Celik, A; Mazmanci, BC; Amlica, YC; Omelekoglu, U; Askin, A
  • The comet assay: what can it really tell us?
    Collins, AR; Dobson, VL; Dusinská, M; Kennedy, G; Stĕtina, R
  • Glutathione metabolism and its role in hepatotoxicity
    DeLeve, LD; Kaplowitz, N
  • Dimethoate-induced oxidative stress and DNA damage in Oncorhynchus mykiss
    Dogan, D; Can, C; Kocyigit, A; Dikilitas, M; Taskin, A; Bilinc, H
  • In vitro effect of malathion and diazinon on oocytes fertilization and embryo development in porcine
    Ducolomb, Y; Casas, E; Valdez, A; Gonzalez, G; Altamirano- Lozano, M; Betancourt, M
  • Biochemical and Histopathological effects of systemic pesticides on some functional organs of male albino rats
    El-Damaty, EM; Farrag, AH; Rowayshed, G; Fahmy, HM
  • Lambda-cyhalothrin-induced changes in oxidative stress biomarkers in rabbit erythrocytes and alleviation effect of some antioxidants
    El-Demerdash, F
  • Sub-chronic exposure to chlorpyrifos induces hematological, metabolic disorders and oxidative stress in rat: attenuation by glutathione
    Elsharkawy, EE; Yahia, D; El-Nisr, N
  • Histopathological and genotoxic effects of chlorpyrifos in rats
    Ezzi, L; Salah, IB; Haouas, Z; Sakly, A; Grissa, I
  • Cytogenetic effects by the natural pyrethrin and the synthetic lambda-cyhalothrin in mice in vivo
    Fahmy, AM; Abdalla, EF
  • Antioxidant systems in insects
    Felton, GW; Summers, CB
  • Oxidative stress induced by lambda-cyhalothrin (LTC) in rat erythrocytes and brain: attenuation by vitamin C
    Fetoui, H; Garoui, M; Makni-Ayadi, F; Zeghal, N
  • Lambda-cyhalothrin-induced biochemical and histopathological changes in the liver of rats: ameliorative effect of ascorbic acid
    Fetoui, H; Garoui, MZ; eghal, N
  • Toxic effects of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the rat kidney: involvement of oxidative stress and protective role of ascorbic acid
    Fetoui, H; Makni, M; Garoui, M; Zeghal, N
  • Differential toxicity of trans-permethrin in rainbow trout and mice. II. Role of target organ sensitivity
    Glickman, AH; Lech, JJ
  • Protective effects of zinc in chlorpyrifos induced hepatotoxicity; a biochemical and trace element study
    Goel, A; Chanuhan, DP; Dhawan, DK
  • Effects of diazinon at different doses on rat liver and pancreas tissues
    Gokcimen, A; Gulle, K; Demirin, H; Bayram, D; Kocak, A; Altuntas, I
  • Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
    Grotto, D; Maria, LS; Valentini, J; Paniz, C; Schmitt, G; Garcia, SC; Pomblum, VJ; Rocha, JB; Farina, M
  • Environmental chemistry, ecotoxicity, and fate of lambda cyhalothrin
    He, L; Troiano, J; Wang, A; Goh, K
  • Protective effect of a synthetic antioxidant “acetyl gallate derivative” against dimethoate induced DNA damage and oxidant antioxidant status in male rats
    Heikal, TM; Mossa, AH; Nawwar, GA; MEl-sherbiny, M; Ghanem, HZ
  • Role of oxidative stress in organophosphate insecticide toxicity—short review
    Hussain, LA
  • Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species
    Kale, M; Rathore, N; John, S; Bhatnagar, D
  • Patho-biochemical studies on hepatotoxicity and nephrotoxicity on exposure to chlorpyrifos and imidacloprid in layer chickens
    Kammon, AM; Brar, RS; Banga, HS; Sodhi, S
  • Single cell gel electrophoresis assay: a new technique for human biomonitoring studies
    Kassie, F; Parzefall, W; Knasmuller, S
  • Toxic effects of chlorpyrifos on antioxidant enzymes and target enzyme acetylcholinesterase interaction in mosquito fish, Gambusia affinis
    Kavitha, P; Rao, JV
  • The agrochemicals handbook
    Kidd, H; James, DR
  • Use of comet and micronucleus assays to measure genotoxicity in meadow voles (Microtus pennsylvanicus) living in golf course ecosystems exposed to pesticides
    Knopper, LD; Mineau, P; McNamee, JP; Lean, DR
  • Pesticide metabolite and oxidative stress in male farmers exposed to pesticide
    Lee, KM; Park, SY; Lee, K; Oh, S; Ko, S
  • DNA damage by iron and hydrogen peroxide in vitro and in vivo
    Linn, S
  • Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms
    Livingstone, DR
  • Haematological and hepatopathological changes induced by dimethoate in Rattus rattus
    Lone, MY; Baba, BA; Raj, P; Shrivastava, VK; Bhide, M
  • Greenfield’s neuropathology
    Lowe, J; Lennox, G; Leigh, PN
  • Hepatoprotective effect of quercetin on lambda-cyhalothrin induced hepatotoxicity in male Wistar rats
    Mani, VM; Ahmed, AN; Ali, AL; Gokulakrishnan, A; Vinoth, K; Hussain, AA
  • Oxidative damage, biochemical and histopathological alterations in rats exposed to chlorpyrifos and the antioxidant role of zinc
    Mansour, SA; Mossa, AT
  • DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring
    Mitchelmore, CL; Chipman, JK
  • Effects of roundup transorb on fish: hematology, antioxidant defenses and acetylcholinesterase activity
    Modesto, KA; Martinez, CB
  • Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion)
    Monteiro, DA; Almeida, JA; Rantin, FT; Kalinin, AL
  • Pesticides and human chronic diseases: evidences, mechanisms and perspectives
    Mostafalou, S; Abdollahi, M
  • Sodium channels and GABAA receptor-channel complex as targets of environmental toxicants
    Narahashi, T; Carter, DB; Frey, J; Ginsburg, K; Hamilton, BJ; Nagata, K; Roy, ML; Song, JH; Tatebayashi, H
  • Evaluation of cytogenetic effects of lambda-cyhalothrin on human lymphocytes
    Naravaneni, R; Jamil, K
  • Different effects of type I and type II pyrethroids on erythrocyte plasma membrane properties and enzymatic activity in rats
    Nasuti, C; Cantalamessa, F; Falcioni, G; Gabbianelli, R
  • Oxidative stress in the blood of farm workers following intensive pesticide exposure
    Ogut, S; Gultekin, F; Kisioglu, AN; Kucukoner, E
  • Greenfield’s neuropathology
    Oppenheimer, DR; Esiri, MM
  • Endometrial damage and apoptosis in rats induced by dichlorvos and ameliorating effect of antioxidant vitamin E and C
    Oral, B; Guney, M; Demirin, H
  • Measuring of effects of pesticides on occupationally exposed humans with the comet assay
    Piperakis, SM; Kontogianni, K; Siffel, C; Piperakis, MM
  • Plasma and RBCs antioxidant status in occupational male pesticide sprayers
    Prakasam, AS; Sethupathy, SL
  • Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite
    Prasanthi, K; MuralidharaRajini, PS
  • Vascular damage mediates neuronal and non-neuronal pathology following short and long-term rotenone administration in Sprague-Dawley rats
    Radad, K; Hassanein, K; Moldzio, R; Rausch, WD
  • A reassessment of the neurotoxicity of pyrethroid insecticides
    Ray, DE; Fry, JR
  • Protective effect of date palm fruit extract (Phoenix dactylifera L.) on dimethoate induced-oxidative stress in rat liver
    Saafi, EB; Louedi, M; Elfeki, A; Zakhama, A; Najjar, MF; Hammami, M; Achour, L
  • Modified toluidine blue: an alternative stain for Helicobacter pylori detection in routine diagnostic use and post-eradication confirmation for gastric cancer prevention
    Sakonlaya, D; Apisarnthanarak, A; Yamada, N; Tomtitchong, P
  • Detection of rodent liver carcinogen genotoxicity by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney and bone marrow)
    Sasaki, YF; Izumiyama, F; Nishidate, E; Matsusaka, N; Tsuda, S
  • Effects of acute dimethoate administration on antioxidant status of liver and brain of experimental rats
    Sharma, Y; Bashir, S; Irshad, M; Gupta, SD; Dogra, TD
  • Pesticides-induced biochemical alterations in occupational North Indian suburban population
    Sharma, RK; Upadhyay, G; Siddiqi, NJ; Sharma, B
  • Erythrocyte antioxidant enzymes in toxicological evaluation of commonly used organophosphate pesticides
    Singh, M; Sandhir, R; Kiran, R
  • Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment
    Soderlund, DM; Clarc, GM; Sheets, LP; Mullin, LS; Piccirillo, VJ
  • Current opinion of the science of organophosphate pesticides and toxic stress: a systematic review
    Soltaninejad, K; Abdollahi, M
  • Comparative toxicity of chlorpyrifos, diazinon, malathion and their oxon derivatives to larval Rana boylii
    Sparling, DW; Fellers, G
  • Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing
    Tice, RR; Agurell, E; Anderson, D; Burlinson, B; Hartmann, A; Kobayashi, H; Miyamae, Y; Rojas, E; Ryu, JC; Sasaki, YF
  • Detection of pyrimethamine-induced DNA damage in mouse embryo and maternal organs by the modified alkaline single cell gel electrophoresis assay
    Tsuda, S; Kosaka, Y; Matsusaka, N; Sasaki, YF
  • Biochemical effects of chlorpyrifos and deltamethrin on altered antioxidative defence mechanisms and lipid peroxidation in rat liver
    Tuzmen, N; Candan, N; Kaya, E; Demiryas, N
  • Neurotoxicological effect and the mode of action of pyrethroid insecticides
    Vijverberg, HP; Bercken, J
  • Interaction of trace metals and different marine food chains
    Wang, WX
  • Effects of atrazine and chlorpyrifos on the mRNA levels of IL-1 and IFN-γ 2b in immune organs of common carp
    Wang, X; Xing, H; Li, X; Xu, S; Wang, X
  • Nerve agents: a comprehensive review
    Wiener, SW; Hoffman, RS

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