Pharm Res (2018) 35: 129 https://doi.org/10.1007/s11095-018-2406-5 RESEARCH PAPER Assessment of Complement Activation by Nanoparticles: Development of a SPR Based Method and Comparison with Current High Throughput Methods 1 1 1,2 Jean-Baptiste Coty & Magali Noiray & Christine Vauthier Received: 21 December 2017 /Accepted: 10 April 2018 /Published online: 26 April 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 ABSTRACT design of the chip proved to give consistent results of comple- Purpose A Surface Plasmon Resonance chip (SPR) was de- ment activation by nanoparticles. veloped to study the activation of complement system trig- gered by nanomaterials in contact with human serum, which . . KEY WORDS automated method complement system is an important concern today to warrant safety of nanoparticles surface plasmon resonance nanomedicines. Methods The developed chip was tested for its specificity in complex medium and its longevity of use. It was then employed to assess the release of complement fragments upon ABBREVIATIONS incubation of nanoparticles in serum. A comparison was made ASTM American Society for Testing and Materials with other current methods assessing complement activation CARPA Complement Activation Related Pseudo Allergy (μC-IE, ELISA). CVF Cobra venom factor Results The SPR chip was found to give
Pharmaceutical Research – Springer Journals
Published: Apr 26, 2018
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