Arsenic-induced changes in growth and antioxidant metabolism of fenugreek

Arsenic-induced changes in growth and antioxidant metabolism of fenugreek The effects of arsenic (As) on growth and antioxidant metabolism of fenugreek (Trigonella foenum-graecum L. cv. Azad) plants were studied using 10, 20, and 30 mg As/kg of soil in a pot experiment under controlled conditions. The length and dry weights of shoots and roots, photosynthetic traits, and grain yield components exhibited a significant increase over control (0 mg As/kg) at As20 but decreased markedly at As30. The cause of this completely reverse response of plant growth between As20 and As30 was investigated in the backdrop of H2O2 metabolism by analyzing responses of three prominent antioxidant enzymes, namely superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) along with cellular ascorbate pool and its redox state. Despite a significant increase in the H2O2 content in both As20 and As30 plants, the former, unlike As30 plants, did not experience any type of As-induced oxidative stress (membrane lipid peroxidation, electrolyte leakage). Normal to high levels of leaf APX, CAT, and redox pool of ascorbate effectively balanced the elevated SOD activity at As20, maintaining the H2O2 concentration higher than control but obviously in favor of As20 plant growth. By contrast, soil amendment with phosphorus (200 mg P/kg) at As30 prevented As-induced oxidative stress through the reduction of the H2O2 level even below As0. The increased enzyme activity was mainly due to the induction of unique Cu/Zn, Fe, and Mn isoforms of SOD and APX-3/APX-4 and/or their increased expression in coordination with CAT. The detection of novel isoforms suggests a strong response of H2O2-metabolizing enzymes against As-induced oxidative stress in fenugreek. Russian Journal of Plant Physiology Springer Journals

Arsenic-induced changes in growth and antioxidant metabolism of fenugreek

Loading next page...
Springer US
Copyright © 2013 by Pleiades Publishing, Ltd.
Life Sciences; Plant Physiology; Plant Sciences
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial