Arch Virol (2007) 152: 219–224
Printed in The Netherlands
Armored RNA as positive control and standard
for quantitative reverse transcription-polymerase
chain reaction assay for rubella virus
, S. Zhao
, and N. Yang
Department of Microbiology, School of Medicine, Shandong University, Jinan, China
Department of Gynecology and Obstetrics, Shandong University, Jinan, China
Department of Laboratory, Tai’an Central Hospital, Tai’an, China
Daan Gene Diagnostic Center, Zhongshan University, Guangzhou, China
Received April 22, 2006; accepted July 13, 2006
Published online September 28, 2006
Summary. RV Armored RNA constructed in the laboratory was shown to be
resistant to RNase and DNase digestion. It could remain stable at 4
C for at
least 60 days, and was much more stable than RV strain JR23 in normal human
plasma. Therefore it could be used as stable, well-characterized positive control
and standard for quantitative RT-PCR assay.
Rubella virus (RV) normally causes a mild, self-limiting disease known as rubella.
Infection with RV during the ﬁrst trimester of pregnancy can induce congenital
malformations known as the congenital rubella syndrome (CRS). Presently, there
is still a signiﬁcant amount of congenitally acquired rubella in some developing
countries and areas [1, 2, 5, 9]. Therefore, a fast, sensitive diagnostic assay is
required for prenatal diagnosis of fetal rubella infection in clinical samples from
pregnant women in cases of suspected rubella infection. The recently developed
real-time quantitative RT-PCR is an increasingly popular technique for rapid and
sensitive quantitation of virus RNA . The key to this quantitative assay is reliable
RNA positive controls and standards.
In this study, RV Armored RNA was constructed by the Armored technology
[3, 4, 7], and then, the emphasis was placed on the possibility of application of RV
Armored RNA as a positive control and standard for TaqMan quantitative RT-PCR
assay for rubella virus.