Aquidulcibacter paucihalophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae isolated from cyanobacterial aggregates in a eutrophic lake

Aquidulcibacter paucihalophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae... A bacterial strain, TH1-2T, was isolated from cyanobacterial aggregates in the eutrophic Lake Taihu, Jiangsu Province, China. Cells were observed to be Gram-negative, slightly curved and rod-shaped. Optimal growth was obtained at pH 7.0 (range 5.5–8.5) and 30 °C (range 20–37 °C) in trypticase soy broth (TSB) without NaCl. Growth was not observed in TSB with 1.0% (w/v) NaCl added. The cells were found to be positive for oxidase and catalase activities. The major fatty acids were identified as 3-hydroxy hexadecanoic acid (C16:0 3-OH), C16:1 ω5c and summed feature 8 (consisting of C18:1 ω6c and cis-11-Octadecenoic acid (C18:1 ω7c). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The 16S rRNA gene sequence of strain TH1-2T was found to be phylogenetically related to those of Glycocaulis abyssi MCS 33T and Brevundimonas naejangsanensis BIO-TAS2-2T (90.5 and 90.0% similarity, respectively). The genomic G+C content of strain TH1-2T was 55.6 mol% based on whole genome calculations. Average nucleotide identities (ANI) and the digital DNA–DNA hybridizations (DDH) for complete genomes ranged from 66.84  to 67.32  and 21.3 to 31.8% between strain TH1-2T and type strains within the family Caulobacteraceae, higher than those between strain TH1-2T and the strains within the family Hyphomonadaceae. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis, indicate that strain TH1-2T (=CGMCC 1.12979T = LMG 28362T) represents a novel species in a new genus within the family Caulobacteraceae; thus, the name Aquidulcibacter paucihalophilus gen. nov., sp. nov., is proposed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Antonie van Leeuwenhoek Springer Journals

Aquidulcibacter paucihalophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae isolated from cyanobacterial aggregates in a eutrophic lake

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Publisher
Springer International Publishing
Copyright
Copyright © 2017 by Springer International Publishing Switzerland
Subject
Life Sciences; Microbiology; Medical Microbiology; Plant Sciences; Soil Science & Conservation
ISSN
0003-6072
eISSN
1572-9699
D.O.I.
10.1007/s10482-017-0889-4
Publisher site
See Article on Publisher Site

Abstract

A bacterial strain, TH1-2T, was isolated from cyanobacterial aggregates in the eutrophic Lake Taihu, Jiangsu Province, China. Cells were observed to be Gram-negative, slightly curved and rod-shaped. Optimal growth was obtained at pH 7.0 (range 5.5–8.5) and 30 °C (range 20–37 °C) in trypticase soy broth (TSB) without NaCl. Growth was not observed in TSB with 1.0% (w/v) NaCl added. The cells were found to be positive for oxidase and catalase activities. The major fatty acids were identified as 3-hydroxy hexadecanoic acid (C16:0 3-OH), C16:1 ω5c and summed feature 8 (consisting of C18:1 ω6c and cis-11-Octadecenoic acid (C18:1 ω7c). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The 16S rRNA gene sequence of strain TH1-2T was found to be phylogenetically related to those of Glycocaulis abyssi MCS 33T and Brevundimonas naejangsanensis BIO-TAS2-2T (90.5 and 90.0% similarity, respectively). The genomic G+C content of strain TH1-2T was 55.6 mol% based on whole genome calculations. Average nucleotide identities (ANI) and the digital DNA–DNA hybridizations (DDH) for complete genomes ranged from 66.84  to 67.32  and 21.3 to 31.8% between strain TH1-2T and type strains within the family Caulobacteraceae, higher than those between strain TH1-2T and the strains within the family Hyphomonadaceae. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis, indicate that strain TH1-2T (=CGMCC 1.12979T = LMG 28362T) represents a novel species in a new genus within the family Caulobacteraceae; thus, the name Aquidulcibacter paucihalophilus gen. nov., sp. nov., is proposed.

Journal

Antonie van LeeuwenhoekSpringer Journals

Published: Jun 9, 2017

References

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