Aptamer-based fluorescence-quenching lateral flow strip for rapid detection of mercury (II) ion in water samples

Aptamer-based fluorescence-quenching lateral flow strip for rapid detection of mercury (II) ion... Divalent mercury ion (Hg2+) is one of the most common and stable forms of mercury pollution. In this study, a skillfully designed lateral flow strip (LFS) was developed for sensitive detection of Hg2+ in river water samples. Aptamer, a specific oligonucleotide probe, was used to selectively identify and target Hg2+ instead of antibody in traditional immunechromatographic strips; and the fluorescence-quenching system was used to generate positive and low background florescence signals in the competitive-likely LFS. The linear detection range of the LFS for Hg2+ was 0.13 ng mL–1 to 4 ng mL–1 and the limit of detection (LOD) was 0.13 ng mL–1. This test provided results in 15 min and demonstrated high specificity. For detection of Hg2+ in river water, the results were consistent with inductively coupled plasma-mass spectrometry measurements. The aptamer-based fluorescence-quenching LFS was shown to provide a reliable, accurate method for rapid detection of mercury contamination. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical and Bioanalytical Chemistry Springer Journals

Aptamer-based fluorescence-quenching lateral flow strip for rapid detection of mercury (II) ion in water samples

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Springer-Verlag GmbH Germany
Subject
Chemistry; Analytical Chemistry; Biochemistry, general; Laboratory Medicine; Characterization and Evaluation of Materials; Food Science; Monitoring/Environmental Analysis
ISSN
1618-2642
eISSN
1618-2650
D.O.I.
10.1007/s00216-017-0491-7
Publisher site
See Article on Publisher Site

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