Arch Virol (2000) 145: 1801–1813
Apricot latent virus: a new species in the genus Foveavirus
L. G. Nemchinov
, A. M. Shamloul
, E. Z. Zemtchik
T. D. Verderevskaya
, and A. Hadidi
Vaccine and Therapeutic Development Section, Oral Infection and Immunity Branch,
National Institute of Dental and Craniofacial Research, National Institutes of Health,
Bethesda, Maryland, U.S.A.
Fruit Laboratory, Agricultural Research Service, U.S. Department of Agriculture,
Beltsville, Maryland, U.S.A.
Research Institute of Horticulture, Chisinau, Moldova
Accepted April 3, 2000
Summary. Extraction of viral double-stranded RNA from peach leaves infected
with Apricot latent virus (ALV) followed by molecular cloning of synthesized
cDNA and its sequencing, suggested that ALV is a new virus, whose coat protein
(CP) coding region contains Apple stem pitting virus (ASPV)-related sequences.
The sequenced portion of the ALV genome (1444 nt) includes the putative CP
gene and the 3
non-translated region. The 5
portion of this fragment (1-651 nt)
is highly distinct whereas the 3
portion is 77% identical to the corresponding
region of ASPV. Molecular hybridization experiments using a cRNA probe to
ASPV with ALV-infected leaf tissue extracts also revealed that the genome of
ALV contains nucleotide sequences related to that of ASPV. Western blots of
tissue extracts indicated that ALV coat protein reacted with polyclonal antiserum
against ASPV; however, the ALV CP differs in size from that of ASPV. ALV was
graft-transmitted to several Prunus rootstocks. Based on the available sequence
data, serologicalobservations andbioassays wepropose thatALV is a new species
in the genus Foveavirus, typiﬁed by ASPV. ALV-speciﬁc PCR-primers and viral-
speciﬁc cRNA probes developed in this investigation may be useful for detecting
the virus and for studying its epidemiology and geographical distribution.
The nucleotide sequence reported in this paper has been deposited at EMBL as
accession number AF057035.
The paper is dedicated to the memory of Tatyana D. Verderevskaya (1927–1999)