Applying an easy molecular method to differentiate Pterois volitans from Pterois miles by RFLPs

Applying an easy molecular method to differentiate Pterois volitans from Pterois miles by RFLPs Numerous papers have reported that Pterois volitans and Pterois miles have invaded the Western Atlantic. Identification of Petrois sp. has been based in morphological differences. However, given their similarities, these are not enough to differentiate both species with certainty. Identifying these species by biological analysis faces difficulties because available methodologies are inaccurate and results unreliable. We developed a method to differentiate these two species using a PCR-RFLPs based protocol. The 476 samples of Pteroides sp. included in the study were collected in the Caribbean and the Gulf of Mexico. To classify them as P. miles or volitans, a DNA sample of each specimen was first analyzed by PCR reaction to amplify cytochrome b (CytB, 1160 bp) gene, followed by digestion of the PCR product using restriction enzymes. Specific banding patterns were visualized for each species by electrophoresis agarose gel. To confirm the presence of these enzyme sites, PCR amplifications of CytB gene were sequenced; resulting sequences were compared with GenBank public database. Our method was 100% effective in the identification of each Pteroids species, providing a quick, easy, and reliable differentiation method. It will help to know the territorial expansion and the basic biology of both species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Conservation Genetics Resources Springer Journals

Applying an easy molecular method to differentiate Pterois volitans from Pterois miles by RFLPs

Loading next page...
 
/lp/springer_journal/applying-an-easy-molecular-method-to-differentiate-pterois-volitans-tSSa0oIr6p
Publisher
Springer Netherlands
Copyright
Copyright © 2017 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Conservation Biology/Ecology; Ecology; Biodiversity; Evolutionary Biology; Plant Genetics and Genomics; Animal Genetics and Genomics
eISSN
1877-7260
D.O.I.
10.1007/s12686-017-0700-x
Publisher site
See Article on Publisher Site

Abstract

Numerous papers have reported that Pterois volitans and Pterois miles have invaded the Western Atlantic. Identification of Petrois sp. has been based in morphological differences. However, given their similarities, these are not enough to differentiate both species with certainty. Identifying these species by biological analysis faces difficulties because available methodologies are inaccurate and results unreliable. We developed a method to differentiate these two species using a PCR-RFLPs based protocol. The 476 samples of Pteroides sp. included in the study were collected in the Caribbean and the Gulf of Mexico. To classify them as P. miles or volitans, a DNA sample of each specimen was first analyzed by PCR reaction to amplify cytochrome b (CytB, 1160 bp) gene, followed by digestion of the PCR product using restriction enzymes. Specific banding patterns were visualized for each species by electrophoresis agarose gel. To confirm the presence of these enzyme sites, PCR amplifications of CytB gene were sequenced; resulting sequences were compared with GenBank public database. Our method was 100% effective in the identification of each Pteroids species, providing a quick, easy, and reliable differentiation method. It will help to know the territorial expansion and the basic biology of both species.

Journal

Conservation Genetics ResourcesSpringer Journals

Published: Feb 16, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off