Review Incorporating Mouse Genome Mammalian Genome 11, 609–613 (2000). © Springer-Verlag New York Inc. 2000 DOI: 10.1007/s003350010119 Applications of DNA microarrays to the transcriptional analysis of mammalian genomes Andy Greenfield MRC Mammalian Genetics Unit, Harwell, Oxfordshire OX11 0RD, UK Received: 15 February 2000 / Accepted: 9 March 2000 Introduction sitivity for a given detection technique given in terms of “x tran- scripts per cell” are often of dubious value). How do these differ- DNA microarrays first gripped the imagination of mammalian ge- ences between unicellular yeast and multicellular mammals affect neticists in 1997 following the publication of two parallel studies the analytical power of microarray technology? Microarrays are on the transcriptional responses of the yeast Saccharomyces cer- intrinsically suited to low-volume hybridizations because they are evisiae to the diauxic shift (DeRisi et al. 1997; Wodicka et al. non-porous, thus aiding the kinetics of association, although the 1997). These studies were remarkable in their scope, each team limits of detection of a fluorescent signal are not as low as that for studying the transcription of virtually every yeast gene simulta- a radioactive equivalent. (The advantages of fluorescent detection neously, in a highly sensitive and quantitative fashion. Patterns of lie in
Mammalian Genome – Springer Journals
Published: Feb 7, 2014
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