ISSN 1063-0740, Russian Journal of Marine Biology, 2008, Vol. 34, No. 1, pp. 38–44. © Pleiades Publishing, Ltd., 2008.
Original Russian Text © Zh.V. Markina, 2008, published in Biologiya Morya.
During the recent decades, sharply negative changes
in ecosystem structure have taken place, biodiversity
was reduced, the number of animals with an unsatisfac-
tory state of health increased, and events of water
blooming became more frequent in the coastal zone of
the Peter the Great Bay. The most important reason for
these phenomena, according to expert opinion, is water
pollution with toxic substances [3, 7, 17, 26, 27, 30].
The most unfavorable ecological situation can be found
near the large thriving port cities of Vladivostok and
Nakhodka , this is why these areas need permanent
monitoring of water conditions.
In estimating water quality, it is necessary to take
into account the peculiarities and tendencies of many
national and international research programs. These
include extension of the role of biological methods in
marine environment research, and biotesting is part of
this trend [5, 7, 28]. Using this method, one can obtain
the integral characteristic of environment quality and
determine the degree of the joint effect of pollutants
[1, 33]. Various organisms can be used for the purpose
of biotesting: ﬁsh, mollusks, crustaceans and higher
plants. However, unicellular algae, due to their small
size, suffer from toxicants more rapidly and to a greater
degree, and this is one of the reasons for their use in
ecotoxicological practice [10, 21, 36]. Microalgae are
convenient to keep in laboratory conditions . As
well, according to Lewis , in 50% of all cases they
are more sensitive to pollutants than animals.
Comparatively resistant green algae are usually
used for biotesting, whereas representatives of other
divisions are used extremely often [36, 38]. Moreno–
Garrido with co-authors  proposed carrying out
water testing using Cryptophyta species, as representa-
tives of this division are sensitive to pollution and abun-
dant in the coastal sea waters. In the summer time they
make the greatest contribution to phytoplankton biom-
ass in the Peter the Great Bay in the Sea of Japan
The purpose of this paper is to investigate the possi-
bility of using the microalga
(Cryptophyta) for the assessment of water quality in the
Amursky and Nakhodka Bays, and to compare this test
(Chlorophyta), which is
frequently used for biotesting.
MATERIAL AND METHODS
Water samples for testing were collected in August
and September 2006 in the Amursky and Nakhodka
Bays (Fig. 1). The names and numbers of stations, as
well as water salinity and pH data are given in the
Table. Sea water salinity was measured using a
GM-65M salinity electrometer; the culture medium pH
was determined using an HI 8314 pH-meter (Hanna).
The unicellular algae
were used as test subjects.
was grown in an
ﬂow nutrient medium , and
in Goldberg medium . Media were prepared using
ﬁltered and pasteurized sea water. Cultivation was car-
ried out in 250 ml Erlenmeyer ﬂasks with a 100 ml vol-
ume of culture medium at 70
s) of luminescent
illumination, a 12 h light : 12 h dark period and at a
Application of the Microalga
for the Assessment of Water Quality in the Amursky
and Nakhodka Bays (Sea of Japan)
Zh. V. Markina
A. V. Zhirmunsky Institute of Marine Biology, Far East Division, Russian Academy of Sciences, Vladivostok, 690041 Russia
Accepted September 13, 2007
—Based on biotesting, we carried out an estimation of the water quality in the Amursky and Nakhodka
Bays (Sea of Japan) using
(Cryptophyta). The obtained data were compared with the
data from water biotesting using
(Chlorophyta). It was shown that water from the Amursky
Bay produced more a negative effect on both microalgae than water from the Nakhodka Bay. It was established
that sensitivity of the
microalga exceeded that of
. This was conﬁrmed by a sharp decrease
motile cell number in the studied water.
, biotesting, water pollution.