Russian Journal of Applied Chemistry, 2013, Vol. 86, No. 8, pp. 1305−1310.
Pleiades Publishing, Ltd., 2013.
Original Russian Text © Yu.A. Shigabieva, S.A. Bogdanova, V.I. Morozov, M.K. Kadirov, Yu.G. Galyametdinov, 2013, published in Zhurnal Prikladnoi Khimii,
2013, Vol. 86, No. 8, pp. 1331−1336.
IN HETEROGENEOUS SYSTEMS
Application of the Electron Paramagnetic Resonance
Method to Determining the Antioxidant Activity
of Structured Colloid Systems
Yu. A. Shigabieva
, S. A. Bogdanova
, V. I. Morozov
, M. K. Kadirov
and Yu. G. Galyametdinov
Kazan National Research Technological University, Kazan, Tatarstan, Russia
Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientiﬁ c Center, Russian Academy of Sciences,
Kazan, Tatarstan, Russia
Received June 25, 2013
Abstract—Possibility of using the electron paramagnetic resonance method to study the antioxidant activity of
structured colloid systems with active components: polymeric cosmetic gels, foaming detergents (shampoos),
and lotions. It is shown that this versatile method opens up new opportunities for determination of the antioxidant
activity not only in liquid-phase systems, but also in gels without any considerable disintegration of their structure.
The antioxidant activity is affected by the concentration of additives, nature of an extract, and composition of
At present, determination of the antioxidant
properties of components in various ﬁ elds of medicine,
cosmetic technology, and medical therapy is of increased
interest because the initiating role of the free-radical
oxidation in a living organism in aging processes has
been accepted . A wide variety of both natural and
synthetic substances are used as antioxidants [2–4].
A topical tendency is the development of treatment-
cosmetic formulations with antioxidants. It has been
shown previously that numerous natural components
(extracts from Chaga mushroom and green tea)
and synthetic substances [N-(β-hydroxyethyl)-4,6-
dimethyldihydropyrimidon-2 (xymedone)] possess
good antioxidant and photoprotective properties [5–8].
In this case, an important task is to develop effective
analytical methods for analysis of the antioxidant
activity. According to the classiﬁ cation suggested
by Khasanov, Ryzhova, and Mal’tseva , there
exist several methods for determining this quantity,
which can be divided into volumetric, photometric,
chemiluminescent, ﬂ uorescent, electrochemical, and a
number of more speciﬁ c techniques [10–14].
The most widely used methods for determining
the antioxidant activity are of indirect type, when
parameters correlated with the antioxidant properties of
active components are recorded. Of particular interest
is a study of the reaction of the ingredient under study
with a stable triarylhydrazyl radical 2,2-diphenyl-
1pycrylhydrazyl (DPPH.) because this radical is stable
in various media in a wide temperature range [15, 16]:
When the free radical interacts with compounds
possessing antioxidant properties, a hydrogen atom is
detached from the antioxidant and transferred to DPPH