Application of H412R mutant alkaline phosphatase for removal of heavy metals from single-ion solutions and effluents

Application of H412R mutant alkaline phosphatase for removal of heavy metals from single-ion... Enzyme-mediated bioremediation is an eco-friendly process for removing hazardous toxic heavy metals from the environ- 2+ 2+ ment. The potential use of mutant alkaline phosphatase H412R for bioprecipitation of heavy metals such as Co , Cd , 6+ 2+ 2+ 2+ Cr, Ni, Mn and Zn from single-ion solutions and electroplating effluents was analysed in the present study. Purified wild-type and H412R mutant alkaline phosphatase enzymes were incubated with an initial concentration of 100 ppm metal solutions for various time periods along with the substrate p-nitrophenol phosphate. Upon catalysis, the enzyme–substrate reaction liberates inorganic phosphate which in turn binds to heavy metals and precipitates them as metal-phosphates. The amount of metal ions precipitated as a result of formation of metal ion-phosphate complexes was determined by estimating the amount of free metal ions present in the solution using atomic absorption spectroscopy. Based on the results obtained, maximum bioprecipitation of metal ions, in general, was observed at 180 min of incubation period. The H412R mutant 2+ 2+ enzyme exhibited higher efficiency and precipitated 96% of Mn from electroplating effluent and 92% of Co from the metal 2+ 6+ 2+ 2+ 2+ 2+ ion solution. The pattern of precipitation of various metal ions was http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Environmental Science and Technology Springer Journals

Application of H412R mutant alkaline phosphatase for removal of heavy metals from single-ion solutions and effluents

Loading next page...
 
/lp/springer_journal/application-of-h412r-mutant-alkaline-phosphatase-for-removal-of-heavy-QPNX5BSSXg
Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Islamic Azad University (IAU)
Subject
Environment; Environment, general; Environmental Science and Engineering; Environmental Chemistry; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution; Soil Science & Conservation; Ecotoxicology
ISSN
1735-1472
eISSN
1735-2630
D.O.I.
10.1007/s13762-018-1730-y
Publisher site
See Article on Publisher Site

Abstract

Enzyme-mediated bioremediation is an eco-friendly process for removing hazardous toxic heavy metals from the environ- 2+ 2+ ment. The potential use of mutant alkaline phosphatase H412R for bioprecipitation of heavy metals such as Co , Cd , 6+ 2+ 2+ 2+ Cr, Ni, Mn and Zn from single-ion solutions and electroplating effluents was analysed in the present study. Purified wild-type and H412R mutant alkaline phosphatase enzymes were incubated with an initial concentration of 100 ppm metal solutions for various time periods along with the substrate p-nitrophenol phosphate. Upon catalysis, the enzyme–substrate reaction liberates inorganic phosphate which in turn binds to heavy metals and precipitates them as metal-phosphates. The amount of metal ions precipitated as a result of formation of metal ion-phosphate complexes was determined by estimating the amount of free metal ions present in the solution using atomic absorption spectroscopy. Based on the results obtained, maximum bioprecipitation of metal ions, in general, was observed at 180 min of incubation period. The H412R mutant 2+ 2+ enzyme exhibited higher efficiency and precipitated 96% of Mn from electroplating effluent and 92% of Co from the metal 2+ 6+ 2+ 2+ 2+ 2+ ion solution. The pattern of precipitation of various metal ions was

Journal

International Journal of Environmental Science and TechnologySpringer Journals

Published: May 31, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off