Application of cationized magnetoferritin for magnetic field-assisted delivery of short interfering RNA in vitro

Application of cationized magnetoferritin for magnetic field-assisted delivery of short... Cationized magnetoferritin is used for development of a simple, efficient, and fast delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetoferritin complex on the surface of plated cells with subsequent application of nanosecond laser pulses producing stress waves in transfection chamber, which permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been quantified using siRNA inducing cell death assay, by combination of these two physical factors we have obtained high efficiency for tested three different human carcinoma cells. Proposed method of gene silencing based on cationized magnetoferritin is a versatile and easily accessible platform with many possible applications in gene therapy. . . . . Keywords Magnetoferritin Interference RNA Cell transfection Magnetophoresis Laser pulses Introduction process are siRNA duplexes (~ 21–23 nt) that are key inter- mediates in the specific degradation of target mRNA follow- Recent advances in the molecular biology of cancer opened ing incorporation into the RNA-induced silencing complex the possibility of cancer gene therapy based on small inter- in the cytosol. Naked siRNA is unstable in blood and serum fering RNA (siRNA). Discovered in Caenorhabditis and is rapidly degraded by nucleases; therefore, several elegans, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Lasers in Medical Science Springer Journals

Application of cationized magnetoferritin for magnetic field-assisted delivery of short interfering RNA in vitro

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Publisher
Springer London
Copyright
Copyright © 2018 by Springer-Verlag London Ltd., part of Springer Nature
Subject
Medicine & Public Health; Medicine/Public Health, general; Dentistry; Optics, Lasers, Photonics, Optical Devices; Quantum Optics
ISSN
0268-8921
eISSN
1435-604X
D.O.I.
10.1007/s10103-018-2547-0
Publisher site
See Article on Publisher Site

Abstract

Cationized magnetoferritin is used for development of a simple, efficient, and fast delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetoferritin complex on the surface of plated cells with subsequent application of nanosecond laser pulses producing stress waves in transfection chamber, which permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been quantified using siRNA inducing cell death assay, by combination of these two physical factors we have obtained high efficiency for tested three different human carcinoma cells. Proposed method of gene silencing based on cationized magnetoferritin is a versatile and easily accessible platform with many possible applications in gene therapy. . . . . Keywords Magnetoferritin Interference RNA Cell transfection Magnetophoresis Laser pulses Introduction process are siRNA duplexes (~ 21–23 nt) that are key inter- mediates in the specific degradation of target mRNA follow- Recent advances in the molecular biology of cancer opened ing incorporation into the RNA-induced silencing complex the possibility of cancer gene therapy based on small inter- in the cytosol. Naked siRNA is unstable in blood and serum fering RNA (siRNA). Discovered in Caenorhabditis and is rapidly degraded by nucleases; therefore, several elegans,

Journal

Lasers in Medical ScienceSpringer Journals

Published: May 30, 2018

References

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