Arch Virol (2005) 150: 407–411
Anulavirus, a proposed new genus of plant viruses
in the family Bromoviridae
D. Gallitelli, M. Finetti-Sialer, and G. P. Martelli
Dipartimento di Protezione delle Piante e Microbiologia Applicata,
Universit`a degli Studi and Istituto di Virologia Vegetale del CNR, Bari, Italy
Received September 16, 2004; accepted October 23, 2004
Published online December 21, 2004
Pelargonium zonate spot virus (PZSV) was ﬁrst isolated in southern Italy from tomato and
tentatively identiﬁed as Tobacco streak virus . It was later found in Pelargonium zonale
plants showing conspicuous concentric chrome-yellow bands in the leaves, from which it
was described and derived its name [9, 5]. New records from tomato came later from Italy
, Spain  and France . The Italian tomato isolate was characterized by Gallitelli .
PZSV has a tripartite genome, particles morphologically similar to those of ilarviruses and
general properties resembling those of tripartite genome viruses of the family Bromoviridae.
Physicochemical and molecular studies have shown that PZSV has speciﬁc properties that
differ enough from those of viruses in other viral species with similar properties described in
the literature [2, 3, 4], to support the likelihood that it should be classiﬁed in a novel taxon.
The establishment of a new genus named Anulavirus (from “anular” Latin for concentric,
because of the type of symptoms shown by its main natural hosts) having PZSV as the type
species was therefore proposed to and approved by the Executive Committee (EC) of ICTV at
its 2004 mid-term meeting in Kingston, Ontario. Formal ratiﬁcation by the full membership
of ICTV will be sought after further consideration by the EC and prior to the next Plenary
Meeting in San Francisco in 2005.
Taxonomic structure of the genus
Anulavirus is a monotypic genus with PZSV as the type species.
In nature, PZSV infects vegetables (tomato, artichoke) and weeds (Capsella bursa-pastoris,
Chrysanthemum segetum, Diplotaxis erucoides, Picreis echiodes, and Sonchus oleraceus).
Experimentally, it has been transmitted by mechanical inoculation to plants in 29 species in
nine dicotyledonous families [4, 8]. PZSV is seed-borne in D. erucoides and Nicotiana gluti-
nosa and thus resembles some ilarviruses. Also, pollen from systemically infected N. glutinosa
was infective when inoculated to test plants and the virus was transmitted from D. erucoides
to tomato in association with pollen grains carried on the bodies of thrips that had fed on its