Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus

Antiviral effects of a probiotic Enterococcus faecium strain against transmissible... The enteropathogenic coronavirus transmissible gastroenteritis virus (TGEV) causes severe disease in young piglets. We have studied the protective effects of the probiotic Enterococcus faecium NCIMB 10415 ( E. faecium ), which is approved as a feed additive in the European Union, against TGEV infection. E. faecium was added to swine testicle (ST) cells before, concomitantly with, or after TGEV infection. Viability assays revealed that E. faecium led to a dose-dependent rescue of viability of TGEV-infected cells reaching nearly to complete protection. Virus yields of the E. faecium –treated cultures were reduced by up to three log 10 units. Western blot analysis of purified TGEV revealed that the levels of all viral structural proteins were reduced after E. faecium treatment. Using transmission electron microscopy, we observed attachment of TGEV particles to the surface of E . faecium which might be a means to trap virus and to prevent infection. Increased production of nitric oxide in the cells treated with E. faecium and elevated expression of interleukin 6 and 8 pointed to stimulated cellular defense as a mechanism to fight TGEV infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-012-1543-0
Publisher site
See Article on Publisher Site

References

  • Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract
    Charteris, WP; Kelly, PM; Morelli, L; Collins, JK
  • A heat labile soluble factor from bacteroides thetaiotaomicron VPI-5482 specifically increases the galactosylation pattern of HT29-MTX cells
    Freitas, M; Cayuela, C; Antoine, JM; Piller, F; Sapin, C; Trugnan, G
  • Host-pathogens cross-talk. Indigenous bacteria and probiotics also play the game
    Freitas, M; Tavan, E; Cayuela, C; Diop, L; Sapin, C; Trugnan, G
  • Probiotics to enhance anti-infective defences in the gastrointestinal tract
    Gill, HS
  • Functional modulation of enterocytes by gram-positive and gram-negative microorganisms
    Otte, JM; Podolsky, DK
  • Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats
    Ruiz, PA; Hoffmann, M; Szcesny, S; Blaut, M; Haller, D
  • Increased litter survival rates, reduced clinical illness and better lactogenic immunity against TGEV in gilts that were primed as neonates with porcine respiratory coronavirus (PRCV)
    Wesley, RD; Lager, KM

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