ISSN 10214437, Russian Journal of Plant Physiology, 2012, Vol. 59, No. 2, pp. 198–205. © Pleiades Publishing, Ltd., 2012.
Published in Fiziologiya Rastenii, 2012, Vol. 59, No. 2, pp. 225–231.
Zinc is an essential element required for all forms
of life. It is now being regarded as the most critical lim
iting nutrient element in crop production after nitro
gen and phosphorus. Indian soils are mostly low in
available Zn levels . Studies of Zn uptake in plants
are critical, as availability of Zn from soils to plants
depends upon several abiotic and biotic factors like
plant species, climate, total available Zn concentra
tion, soil pH, temperature, calcium carbonate,
organic matter, soil texture, microbial activity, salinity,
water logging, and Zn interaction with other elements
like iron, copper, phosphorus, manganese, and mag
nesium and this leads to the problem of Zn deficiency.
To combat this problem, many strategies have been
used from time to time. Among these, production of
Znefficient genotype is a promising strategy, which
can effectively function under low soil Zn condition.
Zincefficient plant genotypes have the ability to grow
better and maintain a higher yield under low Zn in
soil. Nowdays, the use of Znefficient genotypes has
become a sustainable solution of Zn deficiency prob
This text was submitted by the authors in English.
lem. Znefficient plant genotypes reduce additional
fertilizer input and protect the surrounding environ
ment as well . It has been reported that in Zneffi
cient genotypes, the efficiency is an outcome of
enhanced utilization of Zn by the plants. Thus, physi
ological available Zn is an important criterion to judge
Zn efficiency in plants [3–5].
Besides playing a vital role in various metabolic
processes, Zn is known to play a key role in controlling
both ROS generation and detoxification . A
decrease in activities of antioxidant enzymes in Zn
deficient plants have been reported earlier for wheat
[3, 4], beans [7, 8], and rice . However, minimal
information is available regarding the responses of dry
matter accumulation and the antioxidant enzyme
activities in pea genotypes differing in Zn efficiency.
Thus, the objective of the present study was to study
the effect of Zn deficiency on dry matter accumula
tion, tissue Zn and chlorophyll concentrations, and
induction of oxidative stress and antioxidant responses
in two pea genotypes grown under controlled sand cul
ture condition and differing in Zn efficiency.
MATERIALS AND METHODS
In a preliminary experiment, six pea (
L.) genotypes (IPFD9925, IPFD9913,
KPMR500, Rachna, Swati, and Jayanti) procured
from Pulse Research Institute Kanpur were screened
Antioxidant Responses of Pea Genotypes to Zinc Deficiency
N. Pandey, B. Gupta, and G. C. Pathak
Plant Nutrition and Stress Physiology Laboratory, Department of Botany, University of Lucknow, Lucknow, 226007 India;
Received January 5, 2011
—The effects of Zn deficiency on antioxidant responses of two pea (
L.) genotypes, a
Znefficient IPFD9913 and Zninefficient KPMR500, grown in sand culture were studied. In the pea
genotype KPMR500, Zn deficiency decreased dry matter yield, tissue Zn concentration, and antioxidant
enzyme activities istronger than in the genotype IPFD9913. Genotype IPFD9913 developed more effi
cient antioxidant system to scavenge ROS than genotype KPMR500. Zinc deficiency produced oxidative
damage to pea genotypes due to enhanced accumulation of TBARS and H
and decreased activities of
antioxidant enzymes (Cu/Zn superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascor
bate peroxidase (APX)). In the leaves of IPFD9913 genotype, the higher activity of ROSscavenging
enzyme, e.g., SOD, CAT, POD, and glutathione reductase, and antioxidants, such as ascorbate and nonpro
tein thiols, led to the lower accumulation of H
and lipid peroxides. These results suggest that, by main
taining an efficient antioxidant defense system, the IPFD9913 genotype shows a lower sensivity to Zn defi
ciency than the KPMR500 genotype.
Keywords: Pisum sativum
, antioxidant enzymes, Znefficiency.
: APX—ascorbate peroxidase; CA—carbonic anhy
drase; Car—carotenoids; CAT—catalase; Chl—chlorophyll;
Cu/Zn SOD—copper/zinc superoxide dismutase; DHAR—
dehydroascorbate reductase; GR—glutathione reductase;
NPT—nonprotein thiols; POD—peroxidase; TBARS—
thiobarbituric acidreactive substances.