ISSN 10630740, Russian Journal of Marine Biology, 2010, Vol. 36, No. 5, pp. 390–395. © Pleiades Publishing, Ltd., 2010.
Published in Russian in Biologiya Morya.
Carbon tetrachloride is the membranetoxic agent
that is most commonly used in experimental studies
with animals. This toxin first of all affects the mem
branes of the endoplasmic reticulum, where, being
bound with cytochrome P450 as a substrate, it is
metabolized with the formation of free radicals of
trichlormetine and chlorine. Free radicals react with
macromolecules of intracellular and syperoxide
anions membranes and harm their penetrability. This
process is accompanied by hyperproduction of super
oxide anions, which induce lipid peroxidation and
cause oxidative stress .
Complex medications from plants containing
polyphenol compounds are promising for the pharma
cological correction of broken membrane structures.
The medications Carsil, Legalon, Liv 52, and others
created on the basis of the fruit of
Gaerth., which contain silimarine, can be given as
examples . The pharmacodynamics of silimarine
are determined by its membranostabilizing and meta
bolic properties. However the medications that con
tain silimarine are manufactured abroad, which is why
the urgency and necessity of the search for new raw
material sources for the production of medications
with antioxidative and membranoprotective proper
ties on Russian Federation territory are evident.
Papers about the antioxidative properties of the natu
ral polyphenol medication Maksar, which is extracted
[see 2] and developed by the
The article was translated by the authors.
Pacific Institute of Bioorganic Chemistry of the FEB
RAS, have recently been published. The flavonoid
medication Lohein is obtained from
. The therapeutic efficiency of this medication is
conditioned by the inhibition of free radical oxidation
and the intensification of antiradical protection of
membrane lipids, as well as the ability to preserve a
major membrane component, phosphatidylcholine.
But the possibility of using polyphenol complexes
from sea algae as a membranoprotector is discussed
only in single papers [for example, 10]. In this connec
tion we isolated an extract from
containing polyphenol compounds (phlorotannins
and their oligomeric and polymeric forms), polyun
saturated fatty acids of the n3 and n6 families, phos
pholipids, lipophilic substances, aminoacids (there
are seven essential ones among them), proteins, vita
mins, minerals, etc. [4; 10; 16; 20]. Polyphenol com
pounds (phlorotannins and their oligomeric and poly
meric forms) are one of the most important groups of
bioactive substances that create the pharmacologic
value of the algae.
The aim of this work is to investigate the antioxida
tive and membranoprotective properties of an extract
under conditions of carbon
MATERIALS AND METHODS
The brown alga
in 2008 in Zapadnaya Bay of Popov Island (Peter the
Antioxidant and MembraneProtective Properties of an Extract
from the Brown Alga
T. V. Kushnerova
, S. E. Fomenko
, N. F. Kushnerova
, V. G. Sprygin
, L. N. Lesnikova
Yu. S. Khotimchenko
, and E. V. Kondratieva
Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok, 690041 Russia
Pacific Oceanological Institute, Far East Branch, Russian Academy of Sciences, Vladivostok, 690041 Russia
Vladivostok State Medical University, Vladivostok, 690002 Russia
Received January 28, 2010
—This paper presents comparative data on the antioxidant and membraneprotective activities of an
extract from the brown seaweed
and the commercial drug Legalon. It was shown that
intoxication with carbon tetrachloride was accompanied by the exhaustion of the antioxidant defense system
of erythrocytes, an increase in their dimensional characteristics, and a change in the phospholipids to neutral
lipids ratio. The
extract appeared to be more efficient than Legalon in the restoration of the lipid
component of erythrocyte membranes and the removal of toxic stress.
, legalon, carbon tetrachloride, erythrocytes, antioxidant activity, lipids.