ISSN 10227954, Russian Journal of Genetics, 2013, Vol. 49, No. 12, pp. 1190–1194. © Pleiades Publishing, Inc., 2013.
Original Russian Text © O.N. Kurennaya, R.V. Karpova, O.A. Bocharova, I.V. Kazeev, E.V. Bocharov, V.G. Korolev, 2013, published in Genetika, 2013, Vol. 49, No. 12, pp. 1364–1369.
The development of civilization is accompanied by
the increased exposure of the human body to
mutagenic factors from the environment and from
human activities. In this regard, one of the urgent tasks
is to develop genetic safety measures, which include
the identification and further use of antimutagens.
There is a worldwide trend towards intensive study of
antimutagens that act as anticarcinogens [1–3].
Antimutagenic agents can be divided into two
groups, i.e., desmutagens and bioantimutagents.
Desmutagens performed chemical, physical, and bio
chemical modifications of the mutagen, before dam
age in DNA. Bioantimutagens reduce the frequency of
mutations by interaction with the cell and affecting the
processes of cell replication and reparation after DNA
damage. Bioantimutagens include a wide class of
herbal medicinal products with antioxidant, antimu
tagenic, anticarcinogenic, immunomodulatory prop
erties. The antimutagenic properties of many natural
compounds have been established.
The antimutagenic activity of the extract of ginseng
and its individual ginsenosides was revealed in various
experimental models [4–6]. With the
model, the extract of Ginkgo biloba reduced
the level of hydrogen peroxide genotoxicity by stimu
lation of DNA repair . The antimutagenic effect of
lycopene and vitamins C and E was detected using cell
cultures of human carcinomas, defective in correction
of mismatched DNA bases . The genotoxic effect of
cisplatin in mice was less pronounced with the pro
phylactic use of glycyrrhizic acid and the level of DNA
fragmentation and the number of micronuclei were
reduced . Betulinic acid reduced the frequency of
UVinduced mutations in vitro . The reduction of
spontaneous and induced mutagenesis in Saccharo
myces yeast cells was detected under the influence of
hepatoprotector heptronge obtained from defer
mented honey .
Multifitoadaptogene (MPA) developed at the
Immunopharmacology Laboratory of the Blokhin
Cancer Research Center, Russian Academy of Medi
cal Sciences is a preparation based on the components
of extracts of forty plants included in the State Phar
macopoeia of the Russian Federation, including the
wellknown adaptogens of ginseng, rhodiola rosea,
eleuterococcus, magnolia vine, devil’s club, and aralia
. The methods for its biological and chemical
standardization were developed [13–15].
Previous studies have shown antitumor, antioxidant,
immunemodulating properties of MPA [16, 17]. At the
same time, a reduction in the frequency of chromo
some exchanges in lymphocytes of patients with
benign prostatic hyperplasia that resulted from MPA
application was detected .
The goal of this study was to investigate the MPA
effect on the incidence of spontaneous mutations, muta
tions induced by UV radiation and nitrous acid in yeast
Antimutagenesis of Multiphytoadaptogene in Yeast Saccharomyces
O. N. Kurennaya
, R. V. Karpova
, O. A. Bocharova
I. V. Kazeev
, E. V. Bocharov
, and V. G. Korolev
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia
Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, 125315 Russia
Konstantinov St. Petersburg Nuclear Physics Institute, Leningrad oblast, Gatchina, 188300 Russia
Received April 26, 2013
—Multiphytoadaptogene (MPA) consists of plant extracts components including adaptogenes.
Genotoxicity analysis revealed the antimutagenic activity of MPA. MPA decreased the direct mutations fre
loci induced by UV radiation and nitrous acid by 3.7 and 33 times, respectively. The
lethal effect of UV radiation was inhibited when the preparation preparation MFA was used on complete
medium with ethanol. MPA had no effect on replicative mutagenesis. At the same time it depressed mutagen
esis caused by repair errors. The data obtained suggest the antimutagenic activity of multiphytoadaptogene is
associated with postreplicative repair activation.
GENETICS OF MICROORGANISMS