Antibiotic gene specific characterization and ARDRA analysis of native isolates of Pseudomonas spp. from Jammu, India

Antibiotic gene specific characterization and ARDRA analysis of native isolates of Pseudomonas... The genus Pseudomonas is well known biocontrol agent established for its diverse role and antagonistic properties against phytopathogens due its ability of producing antibiotics. The studies were conducted to characterize Pseudomonas spp. for antibiotic production on the basis of gene specific primer. 150 isolates of Pseudomonas spp. were isolated from different agricultural fields of Jammu on King’s B medium using plate dilution method. Out of the total, thirty isolates were selected. The antibiotic i.e. 2,4-Diacetylphloroglucinol (2,4-DAPG) extracted from the various isolates showed spots having R values in the range of 0.7–0.89 which is very close to the R value of synthetic phloroglucinol (0.89). 2,4-DAPG positive isolates were tested against Fusarium oxysporum using dual culture assay and they were found to inhibit the mycelial growth of F. oxysporum by 52.76–65.45%. PCR analysis with 2,4-DAPG gene (phlD) specific primer indicated that a DNA fragment of approximately 750 bp in size was obtained in all the strains except PS 3 and 5 which is closely corroborated with the already reported size of 2,4-DAPG gene in literature. This confirms that 28 isolates out of 30 isolates have the genetic ability for the production of 2,4-DAPG antibiotic and thus these isolates can be promoted as http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Indian Phytopathology Springer Journals

Antibiotic gene specific characterization and ARDRA analysis of native isolates of Pseudomonas spp. from Jammu, India

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Publisher
Springer India
Copyright
Copyright © 2018 by Indian Phytopathological Society
Subject
Life Sciences; Plant Pathology
ISSN
0367-973X
eISSN
2248-9800
D.O.I.
10.1007/s42360-018-0028-9
Publisher site
See Article on Publisher Site

Abstract

The genus Pseudomonas is well known biocontrol agent established for its diverse role and antagonistic properties against phytopathogens due its ability of producing antibiotics. The studies were conducted to characterize Pseudomonas spp. for antibiotic production on the basis of gene specific primer. 150 isolates of Pseudomonas spp. were isolated from different agricultural fields of Jammu on King’s B medium using plate dilution method. Out of the total, thirty isolates were selected. The antibiotic i.e. 2,4-Diacetylphloroglucinol (2,4-DAPG) extracted from the various isolates showed spots having R values in the range of 0.7–0.89 which is very close to the R value of synthetic phloroglucinol (0.89). 2,4-DAPG positive isolates were tested against Fusarium oxysporum using dual culture assay and they were found to inhibit the mycelial growth of F. oxysporum by 52.76–65.45%. PCR analysis with 2,4-DAPG gene (phlD) specific primer indicated that a DNA fragment of approximately 750 bp in size was obtained in all the strains except PS 3 and 5 which is closely corroborated with the already reported size of 2,4-DAPG gene in literature. This confirms that 28 isolates out of 30 isolates have the genetic ability for the production of 2,4-DAPG antibiotic and thus these isolates can be promoted as

Journal

Indian PhytopathologySpringer Journals

Published: Jun 6, 2018

References

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