Anther Culture Response of Barley Genotypes to Cold Pretreatments and Culture Media

Anther Culture Response of Barley Genotypes to Cold Pretreatments and Culture Media The embryoid formation and plant regeneration in anther cultures of three barley (Hordeum vulgare L.) cultivars (Niki, Karina, Thermi), one F1 hybrid (Niki × Thermi), two F2 populations (Niki × Thermi, Niki × Karina), and two F3 populations (Niki × Thermi, Niki × Karina) were investigated in two solid induction media after cold pretreatment for 14 and 28 days at 4°C . The media used (N6 and FHG) differed in their composition and source of energy (maltose in FHG vs. sucrose in N6). Embryoid frequency and green plant regeneration depended on both the induction medium composition and cold pretreatment. The combination of the FHG induction medium with 28-day-long cold pretreatment was the most efficient in haploid embryoid formation and green plant production. In addition, the green plant production was genotype-dependent. Cv. Thermi and F1 hybrid Niki × Thermi exhibited the highest frequency of green plant production. The parent with high or even moderate frequency of embryoid formation in anther culture could lead to the effective production of green plants from the F1 hybrid or the F2 generation for breeding purposes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Anther Culture Response of Barley Genotypes to Cold Pretreatments and Culture Media

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2005 by MAIK "Nauka/Interperiodica"
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1007/s11183-005-0104-8
Publisher site
See Article on Publisher Site

Abstract

The embryoid formation and plant regeneration in anther cultures of three barley (Hordeum vulgare L.) cultivars (Niki, Karina, Thermi), one F1 hybrid (Niki × Thermi), two F2 populations (Niki × Thermi, Niki × Karina), and two F3 populations (Niki × Thermi, Niki × Karina) were investigated in two solid induction media after cold pretreatment for 14 and 28 days at 4°C . The media used (N6 and FHG) differed in their composition and source of energy (maltose in FHG vs. sucrose in N6). Embryoid frequency and green plant regeneration depended on both the induction medium composition and cold pretreatment. The combination of the FHG induction medium with 28-day-long cold pretreatment was the most efficient in haploid embryoid formation and green plant production. In addition, the green plant production was genotype-dependent. Cv. Thermi and F1 hybrid Niki × Thermi exhibited the highest frequency of green plant production. The parent with high or even moderate frequency of embryoid formation in anther culture could lead to the effective production of green plants from the F1 hybrid or the F2 generation for breeding purposes.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Sep 28, 2005

References

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