Anion Modulation of the Slowly Activating Vacuolar Channel

Anion Modulation of the Slowly Activating Vacuolar Channel This study addresses the permeability and modulation by anions of the slowly-activating vacuolar (SV) channel in the tonoplast of Mesembryanthemum crystallinum. Although we conclude that the M. crystallinum SV channel is impermeable to anions, the anion species at the cytosolic side of the membrane clearly affects the channel. We discuss two types of modulation, both consistent with a single binding site for anions at the cytosolic face of the channel. The first effect is related to the conducting properties of SV. When the site is occupied, the bound anion exerts an effect on the Ca2+ and Na+ fluxes through the channel. Because the Stokes-diameter of both ion species is different, both fluxes are not influenced to the same extent. This differential effect on the permeation of both ion species is reflected in a permeability ratio Ca2+ over Na+ (P Ca/P Na) that depends on the identity of the anion species. The second type of modulation deals with the activation of the channel by anions. Previous studies revealed that a low level of cytosolic Cl− inactivates SV (Pantoja, Dainty & Blumwald, 1992a). We critically evaluated reported effects of anions on the whole-vacuole SV current. Given that cytosolic Ca2+ and Cl− both modulate SV, we conclude that both levels of regulation show some kind of interaction. High cytosolic Ca2+ can overrule the inactivation caused by low cytosolic Cl−. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Anion Modulation of the Slowly Activating Vacuolar Channel

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 2001 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-001-0061-x
Publisher site
See Article on Publisher Site

Abstract

This study addresses the permeability and modulation by anions of the slowly-activating vacuolar (SV) channel in the tonoplast of Mesembryanthemum crystallinum. Although we conclude that the M. crystallinum SV channel is impermeable to anions, the anion species at the cytosolic side of the membrane clearly affects the channel. We discuss two types of modulation, both consistent with a single binding site for anions at the cytosolic face of the channel. The first effect is related to the conducting properties of SV. When the site is occupied, the bound anion exerts an effect on the Ca2+ and Na+ fluxes through the channel. Because the Stokes-diameter of both ion species is different, both fluxes are not influenced to the same extent. This differential effect on the permeation of both ion species is reflected in a permeability ratio Ca2+ over Na+ (P Ca/P Na) that depends on the identity of the anion species. The second type of modulation deals with the activation of the channel by anions. Previous studies revealed that a low level of cytosolic Cl− inactivates SV (Pantoja, Dainty & Blumwald, 1992a). We critically evaluated reported effects of anions on the whole-vacuole SV current. Given that cytosolic Ca2+ and Cl− both modulate SV, we conclude that both levels of regulation show some kind of interaction. High cytosolic Ca2+ can overrule the inactivation caused by low cytosolic Cl−.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Sep 15, 2001

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