ISSN 1022-7954, Russian Journal of Genetics, 2007, Vol. 43, No. 2, pp. 149–155. © Pleiades Publishing, Inc., 2007.
Original Russian Text © A.M. Shimkevich, N.V. Lukhanina, I.M. Goloenko, O.G. Davydenko, 2007, published in Genetika, 2007, Vol. 43, No. 2, pp. 209–216.
Numerous facts today demonstrate the ability of
cytoplasmic genomes in the plant cell to inﬂuence the
formation and development of certain productivity
traits, resistance to diseases, and tolerance of adverse
environmental factors [1–3].
The genomes of organelles and nucleus are capable
of mutually inﬂuencing various genetic processes. For
example, it is known that the equal probability of seg-
regation of dominantand recessive alleles and their
transmission to the progeny of heterozygous organisms
and, as a result, the predictability of their segregation,
is a fundamental principle of the Mendelian genetics.
However, there is a phenomenon known as segregation
distortion (SD) or meiotic drive. Examples of this phe-
nomenon are the
of mice, plant B chromosomes, spore killer in fungi,
and others [4–6]. A number of segregation distortion
instances in morphological, biochemical, and molecu-
lar markers are also known for barley [7, 8]. Moreover,
the degree of deviation, measured by chi square param-
eter, varies from small signiﬁcant values within the
norm limit to considerable values . Small segrega-
tion distortions are frequently met; however, many
researchers ignore them. The question on whether a
small segregation distortion is rather a rule than a devi-
ation is yet unanswered . The fraction of the traits
displaying the distortion also varies, reaching fre-
quently 40% [10–12].
The study into speciﬁc features of the inﬂuence that
organelle genomes exert on transmission and recombi-
nation of the nuclear genes playing an important role in
the development of plant productivity as well as in the
population processes and evolution is extremely neces-
sary for understanding of the mechanisms underlying
the interactions between nuclear and organelle
genomes. In addition, such research may bring us
closer to the possibility of regulating the recombination
and directed transmission of nuclear genes in the pres-
ence of different plasmatypes.
A large number of works demonstrating the occur-
rence of segregation distortion in plants appeared after
the advent of molecular markers in genetic research.
This approach expands considerably our concepts of
the structure–function organization of organelle
genomes and brings us closer to understanding of the
processes involved in the interactions between the
nucleus and the cytoplasm.
Earlier our laboratory bred a collection of barley
alloplasmic lines, which combines seven nuclear (
) and twelve cytoplasmic (
) genomes. Restriction analysis demonstrated that
the wild forms of
) used as the donors of
cytoplasm differed in their chloroplast and/or mito-
chondrial DNAs, forming several plasmatypes [13, 14].
It was also shown that various cytoplasms were capable
of inﬂuencing differently the development of produc-
tivity traits in alloplasmic lines .
In this work, we studied the transmission and
recombination of three microsatellite loci in barley
linkage group 5H(7) as well as several morphological
marker loci localized to different chromosomes in the
presence of various cytoplasmic genomes.
MATERIALS AND METHODS
Six pairs of barley reciprocal hybrid populations
obtained using the alloplasmic lines bred by I.M. Golo-
enko were the material for the study into the effect of
Analysis of the Segregation and Recombination Rates
at Morphological and SSR Loci in Hybrid Combinations
of Barley Substitution Lines
A. M. Shimkevich, N. V. Lukhanina, I. M. Goloenko, and O. G. Davydenko
Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, 220141 Belarus;
fax: (375) 17 263-58-27; e-mail: firstname.lastname@example.org
Received March 10, 2006
—The inheritance of nuclear morphological and molecular markers was studied in hybrid reciprocal
populations of barley alloplasmic lines. A number of loci displayed a distortion of the Mendelian inheritance
and a decrease in recombination rate depending on the cross direction and cytoplasm of parental forms. The
segregations shifted towards both the mutant recessive and normal dominant phenotypes. The effect of cyto-
plasm on transmission and recombination of nuclear loci was shown.