Analysis of the morphogenetic cluster and genome of the temperate Lactobacillus casei bacteriophage A2

Analysis of the morphogenetic cluster and genome of the temperate Lactobacillus casei... The genes that encode the morphogenetic proteins of bacteriophage A2 are clustered and expressed as a single operon which originates a late transcript of more than 20 kb. This DNA stretch is analyzed in the context of the whole phage genome, which presents the following peculiarities: a) the head presents two major proteins that share their NH 2 termini, i.e.: both are translated from a single gene ( orf5 ), b) these two proteins suffer a proteolytic maturation process before being incorporated into the capsid, rendering a 123 NH 2 terminal putative polypeptide that is postulated to be the scaffolding protein of the phage, c) similar maturation processes occur at the portal and tail length determination proteins, having all in common a Pho-Pho-Arg↓ sequence (where Pho stands for any hydrophobic amino acid) at the processing point, d) the genes encoding the subunits of the terminase ( orf61 and orf2 ) are separated by the cohesive ends, e) two genes that might mediate lysogenic conversion ( orf19 and orf22 ) have been identified and f) the genome presents a dispensable region (which covers at least 10 orfs , as judged from analysis of deletion mutants) that might be involved in maintaining its size between the packaging limits of the capsid. Archives of Virology Springer Journals

Analysis of the morphogenetic cluster and genome of the temperate Lactobacillus casei bacteriophage A2

Loading next page...
Copyright © 2002 by Springer-Verlag/Wien
Publisher site
See Article on Publisher Site

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial