ISSN 10227954, Russian Journal of Genetics, 2010, Vol. 46, No. 12, pp. 1462–1470. © Pleiades Publishing, Inc., 2010.
Original Russian Text © Yu.A. Stolpovsky, N.V. Kol, A.N. Evsyukov, M.N. Ruzina, L.V. Shimiit, G.E. Sulimova, 2010, published in Genetika, 2010, Vol. 46, No. 12, pp. 1660–1669.
The use of DNA markers obtained by the poly
merase chain reaction (PCR) with primers having
multiple localization in the genome is one of efficient
approaches in different fields of molecular, popula
tion, conservation, and special genetics. The study of
genomes of domesticated species with such methods is
of fundamental and practical interest . The ISSR
assay makes it possible to reveal inter and intraspe
cific polymorphism, ecological and genetic relations,
to differentiate plant and animal species, and estimate
their genetic diversity and consolidation [2–6]. Anal
ysis of length polymorphism of DNA intermicrosatel
lite sequences has a number of apparent advantages.
ISSRPCR is a relatively inexpensive and simple
express method and does not require knowledge of the
genome nucleotide sequence, which is important in
studying the gene pools of poorly known species. This
method permits a simultaneous testing of tens of mic
rosatellite loci represented mainly by unique DNA
sequences surrounding or being a part of many genes,
which correspondingly allows searching for correla
tions with the phenotypical characters and estimating
the level of polymorphism in the genome as a whole.
The intermicrosatellite analysis showed that the distri
bution of the spectrum of PCR products obtained with
the use of primers for di and trinucleotide repeats is
specific and highly informative in humans, plants, and
wild and domesticated animals [2–6].
We chose a unique ancient local breed of the Tuvin
ian shortfattailed sheep as one of the breeds that are
of prime interest for conservation of genetic resources
of domesticated species .
The tasks of this study were to identify breedspe
cific fragments and patterns, to determine the gene
pool profile (combination of genotypes for different
loci) and the gene pool standard of the breed, and to
reveal flocks of sheeps that are most typical of the
breed. On the basis of this material, we explored the
possibilities of using ISSR markers in the control and
monitoring of the genetic structure of the populations
and in establishing genealogical relations between
MATERIALS AND METHODS
Brief characterization of the breed.
shortfattailed sheep is a valuable local breed, which
have been developed during no less than 3000 years. It
was bred by tribes inhabiting the territory of modern
Tyva and is very well adapted to the natural climatic
and pasture conditions and to the nomadic life style of
the local population. The Tuvinian sheep was bred by
folk selection and is an object of traditional husbandry.
Because of the geographic isolation of a greater terri
tory of Tyva, the Tuvinian sheeps were subjected to dif
ferent crosses to a lesser extent than other local breeds.
Nevertheless, attempts are known to improve the
Tuvinian sheep, which were aimed at increasing its
wool productivity through crosses with finewooled
breeds (in particular, with the Krasnoyarsk, Altai, and
Karakul’ breeds, with Novocaucasian merinos, and
even with American rambouillet rams . The Tuvin
Analysis of the Genetic Structure of Tuvinian ShortFatTailed
Sheep Populations with the Use of the ISSRPCR Method
Yu. A. Stolpovsky
, N. V. Kol
, A. N. Evsyukov
, M. N. Ruzina
L. V. Shimiit
, and G. E. Sulimova
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia;
Tuvinian State University, Department of Zootecnics, Tyva, Kyzyl, 667000 Russia
Received July 23, 2009
—The genetic structure of populations of the Tuvinian shortfattailed sheep was studied with the
use of the ISSRPCR (Inter Simple Sequence Repeats) method in 18 farms of Tyva. Data on the spectrum of
ISSR fragments of DNA were obtained using the (AG)
C primer. Analysis of intermicrosatellite polymor
phism permitted us to determine genomic characteristics of the populations, their genealogical relations, and
the parameters of genetic diversity within the populations and the breed as a whole. Three genetic notions
were considered on the basis of the results of this analysis: gene pool profile, gene pool standard, and breed
specific pattern. The data obtained can be used to carry out population genetic monitoring, to develop a
breeding strategy, and to conserve in situ the Tuvinian sheep breed and breeds of other domesticated species.