Analysis of the gamete-fusion genes in the haploid-inducing ZMS-P maize line

Analysis of the gamete-fusion genes in the haploid-inducing ZMS-P maize line This article is devoted to the study of the double fertilization mechanism in plants, in particular of the maize gamete membrane fusion genes. We detected and analyzed for the first time gamete-fusion genes in the maize genome. Using the BLAST program, we searched for the hap2 gene (generative cell specific 1 (gcs1)) homologs from Arabidopsis in the maize genome. The ZM_BFb0162K03 maize transcript was found, which had 67% identity to the Athap2 gene and contained a conserved region similar to the Athap2 gene fragment. In mRNA samples from the haploid-inducing and control maize lines, an PCR was conducted by using primers specific to the ZM_BFb0162K03 sequence fragment. Sequences of the PCR products from a fragment (1467 bp) of the Zm_hap2 gene of the haploid-inducing and the control maize lines were identical and also were identical to the maize sequences from the GenBank (ZM_BFb0162K03). PCR products (656 bp region of Zm_hap2) for the ZM_BFb0162K03 (1925 bp) maize sequence were observed for the cDNA of pollen grains, ovary, leaves, and roots of the haploid-inducing and control maize lines. Using the Blastx program, we found significant homology of the maize translated proteins to the GEX2, TET11, and TET12 proteins, involved in Arabidopsis gamete-fusion contacts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Analysis of the gamete-fusion genes in the haploid-inducing ZMS-P maize line

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Publisher
Pleiades Publishing
Copyright
Copyright © 2017 by Pleiades Publishing, Inc.
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360417020096
Publisher site
See Article on Publisher Site

Abstract

This article is devoted to the study of the double fertilization mechanism in plants, in particular of the maize gamete membrane fusion genes. We detected and analyzed for the first time gamete-fusion genes in the maize genome. Using the BLAST program, we searched for the hap2 gene (generative cell specific 1 (gcs1)) homologs from Arabidopsis in the maize genome. The ZM_BFb0162K03 maize transcript was found, which had 67% identity to the Athap2 gene and contained a conserved region similar to the Athap2 gene fragment. In mRNA samples from the haploid-inducing and control maize lines, an PCR was conducted by using primers specific to the ZM_BFb0162K03 sequence fragment. Sequences of the PCR products from a fragment (1467 bp) of the Zm_hap2 gene of the haploid-inducing and the control maize lines were identical and also were identical to the maize sequences from the GenBank (ZM_BFb0162K03). PCR products (656 bp region of Zm_hap2) for the ZM_BFb0162K03 (1925 bp) maize sequence were observed for the cDNA of pollen grains, ovary, leaves, and roots of the haploid-inducing and control maize lines. Using the Blastx program, we found significant homology of the maize translated proteins to the GEX2, TET11, and TET12 proteins, involved in Arabidopsis gamete-fusion contacts.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Apr 5, 2017

References

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