Analysis of NaoMaiTong Metabolites Using High-Performance Liquid Chromatography/High-Resolution Mass Spectrometry in Rat Urine

Analysis of NaoMaiTong Metabolites Using High-Performance Liquid Chromatography/High-Resolution... Chromatographia (2017) 80:1371–1399 DOI 10.1007/s10337-017-3363-6 ORIGINAL Analysis of NaoMaiTong Metabolites Using High‑Performance Liquid Chromatography/High‑Resolution Mass Spectrometry in Rat Urine 1 1 1 Xulei Fan  · Yueying Rong  · Shumei Wang   Received: 29 April 2017 / Revised: 13 July 2017 / Accepted: 17 July 2017 / Published online: 29 July 2017 © Springer-Verlag GmbH Germany 2017 Abstract NaoMaiTong (NMT), consisting of Radix et Rhi- presented different absorption trends and slower elimination zoma Rhei, Radix Ginseng, Radix Puerariae, and Rhizoma rates in the urine from the NMT-dosed group compared with Ligustici Chuanxiong, is widely used for treating ischemia the urine from the single-dosed groups. These results will cerebral apoplexy. In this work, a rapid high-performance liq- provide helpful information for further basic research into the uid chromatograph coupled with a quadrupole-orbitrap mass active substances in NaoMaiTong. spectrometer (HPLC-Q-Orbitrap) was developed for detec- tion and identification of chemical compounds in rat urine Keywords HPLC-Q-Orbitrap · Metabolites · Metabolic after oral administration of NaoMaiTong and its single herbs. pathways · NaoMaiTong Using targeted screening and the mass defect filter of Met - Works™ software, a total of 157 ingredients were detected in the NaoMaiTong drug-containing group within 36 h, includ- Introduction ing 70 prototype chemicals and 61 related metabolites that were Chromatographia Springer Journals

Analysis of NaoMaiTong Metabolites Using High-Performance Liquid Chromatography/High-Resolution Mass Spectrometry in Rat Urine

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Springer Berlin Heidelberg
Copyright © 2017 by Springer-Verlag GmbH Germany
Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine; Analytical Chemistry
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