In this study, Candida tropicalis, Aspergillus niger, and a mixture of their cultures were previously used as fungal elicitors in Panax quinquefolium adventitious root culture to enhance ginsenoside production. The result showed that ginsenoside content (11.47 ± 0.54 mg g−1) was significantly enhanced to 3.25-fold of the control group after treatment with 400 mg L−1 C. tropicalis. The polysaccharide content (213.01 ± 18.63 mg g−1) reached the highest when A. niger was at the concentration of 200 mg L−1. HPLC–ESI-MSn analysis was performed, showing that ginsenosides Rh1 and Ro were identified after treatment with fungal elicitors. Furthermore, we found that C. tropicalis, A. niger, and mix elicitor significantly up-regulated the expression of the squalene synthase, squalene epoxidase, dammarenediol synthase, β-amyrin synthase, protopanaxadiol synthase, protopanaxatriol synthase, and UDP-glycosyltransferase. SDS-polyacrylamide gel electrophoresis was used to analysis the changes of protein. A new band was discovered at the size of 75 kDa. In addition, it found that fungal elicitors significantly increased the activities of catalases and peroxidase.
Research on Chemical Intermediates – Springer Journals
Published: Oct 17, 2016
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