Analysis of gene functions by a syringe infiltration method of VIGS

Analysis of gene functions by a syringe infiltration method of VIGS Virus-induced gene silencing (VIGS) is a powerful tool for characterization of functional genes in tomato. In this study, we improved the syringe infiltration method on detached tomato fruits. With PDS (phytoene desaturase gene) used as a reporter gene, harvested mature green tomato fruits were syringe-infiltrated with agrobacterium strain GV3101 containing pTRV1 and pTRV2-LePDS, which resulted in color changing on the fruit surface. The changes in pigments and transcript abundance of PDS were compared between silencing sectors and control. In addition, factors of environmental conditions, such as temperature and humidity, were optimized for gene silencing, and other factors, including volume of agrobacterial culture, were also analyzed. Furthermore, this silencing method was confirmed by silencing LeRIN and LeACS2, which influenced fruit ripening process as positively regulating genes, and LeCTR1, which took part in ethylene signal transduction as a negatively regulating gene. Transcripts of LeRIN, LeCTR1, and LeACS2 were measured in the following analysis of gene silencing. All results indicated that an infiltration-based method is efficient for gene characterization during development and ripening of tomato fruits. Russian Journal of Plant Physiology Springer Journals

Analysis of gene functions by a syringe infiltration method of VIGS

Loading next page...
SP MAIK Nauka/Interperiodica
Copyright © 2010 by Pleiades Publishing, Ltd.
Life Sciences; Plant Sciences ; Plant Physiology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial