Arch Virol (2004) 149: 1643–1652
Analysis of an isolate of Mungbean yellow mosaic virus
(MYMV) with a highly variable DNA B component
A. S. Karthikeyan
, R. Vanitharani
, V. Balaji, S. Anuradha,
P. Thillaichidambaram, P. V. Shivaprasad, C. Parameswari,
V. Balamani, M. Saminathan, and K. Veluthambi
Centre for Plant Molecular Biology and Department of Plant Biotechnology,
School of Biotechnology, Madurai Kamaraj University, Madurai, India
Received July 14, 2003; accepted February 2, 2004
Published online April 5, 2004
Summary. One DNA A (KA30) and ﬁve different DNA B components (KA21,
KA22, KA27, KA28 and KA34) of a geminivirus, Mungbean yellow mosaic
virus–Vigna (MYMV-Vig) were cloned from a pooled sample of ﬁeld-infected
Vigna mungo plants from Vamban, South India. MYMV-Vig DNA A (KA30)
and one of the DNA B components (KA27) exhibited 97% and 95% sequence
identities, respectively, to those of MYMV reported from Thailand. However,
the DNA B components KA21, KA22, KA28 and KA34 exhibited only 71 to
72% sequence identity to MYMV DNA B. Co-existence of multiple DNA B
components in ﬁeld-infected V. mungo was proved by Southern and PCR analyses.
Each of the ﬁve DNA B components was infective together with the DNA A
upon agroinoculation. Agroinoculation with mixed cultures of Agrobacterium
with partial dimers of DNA A and all ﬁve DNA Bs proved that all ﬁve DNA B
components can co-infect a single V. mungo plant.
Mungbean yellow mosaic virus (MYMV) infects ﬁve important pulse crops, black-
gram, mungbean, Frenchbean, pigeonpea and soybean, causing an annual yield
Note: The nucleotide sequences reported in this paper have been deposited in the
EMBL/GenBank/DDJB Nucleotide Sequence Database under the accession numbers
AJ132575 (DNA A), AJ439059 (KA21 DNA B), AJ132574 (KA22 DNA B), AF262064
(KA27 DNA B), AJ439058 (KA28 DNA B) and AJ439057 (KA34 DNA B).
A. S. K. and R. V. contributed equally to this work.