Analysis of a translational enhancer present within the 5′-terminal sequence of the genomic RNA of potato virus S

Analysis of a translational enhancer present within the 5′-terminal sequence of the genomic RNA... When present as a transcript leader the 5′ untranslated sequence from the potato virus S genomic RNA molecule enhances translation of a downstream open reading frame both in vitro and in vivo. Translational enhancement was 30-fold in rabbit reticulocyte lysate and 15 fold in wheat germ above translation from a transcript with a synthetic leader. Transient expression experiments using tobacco protoplasts and particle bombardment of leaf tissue resulted in enhancement of fourteen and five-fold, respectively, above translation with a synthetic leader. In stably transformed plants the PVS 5′UTR enhanced translation yield ca. 5-fold compared with a synthetic 5′UTR. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Analysis of a translational enhancer present within the 5′-terminal sequence of the genomic RNA of potato virus S

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Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1999 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050601
Publisher site
See Article on Publisher Site

Abstract

When present as a transcript leader the 5′ untranslated sequence from the potato virus S genomic RNA molecule enhances translation of a downstream open reading frame both in vitro and in vivo. Translational enhancement was 30-fold in rabbit reticulocyte lysate and 15 fold in wheat germ above translation from a transcript with a synthetic leader. Transient expression experiments using tobacco protoplasts and particle bombardment of leaf tissue resulted in enhancement of fourteen and five-fold, respectively, above translation with a synthetic leader. In stably transformed plants the PVS 5′UTR enhanced translation yield ca. 5-fold compared with a synthetic 5′UTR.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 1999

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